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Product Info Summary
| SKU: | A04422 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Twist2 Antibody
SKU/Catalog Number
A04422
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Twist2 Antibody (Catalog # A04422). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Twist2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04422)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.26~30(P-K-R-F-G) derived from Human Twist2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04422 is reactive to Twist2 in Human, Mouse, Rat
Applications
A04422 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
18.14kDa
Background of Twist-2
Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism. Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting 1:100-1:500
Immunohistochemistry 1:100-1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from A549, Hela and 3T3 cells using Twist2 Antibody #A04422.
Protein Target Info & Infographic
Gene/Protein Information For Twist2 (Source: Uniprot.org, NCBI)
Gene Name
Twist2
Full Name
Twist-related protein 2
Weight
18.14kDa
Alternative Names
bHLHa39; bHLHa39twist-related bHLH protein Dermo1; Class A basic helix-loop-helix protein 39; Dermis-expressed protein 1; DERMO1; Dermo-1; DERMO1MGC117334; twist homolog 2 (Drosophila); Twist2; Twist-2; twist-related protein 2 Twist2|Derm, Dermo1, bHLHa, bHLHa39|twist basic helix-loop-helix transcription factor 2|twist-related protein 2|dermis-expressed protein 1|twist homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Twist2, check out the Twist2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Twist2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Twist2 Antibody (A04422)
Hello CJ!
No publications found for A04422
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-Twist2 Antibody
Question
My question regarding product A04422, anti-Twist2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04422 anti-Twist2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-17
Question
See attached the WB image, lot number and protocol we used for pubmed:11062344} using anti-Twist2 antibody A04422. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-15
Question
Will anti-Twist2 antibody A04422 work on feline WB with endocervix?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
Our lab technicians have not validated anti-Twist2 antibody A04422 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Twist2 antibody A04422 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline endocervix in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-01
Question
Is a blocking peptide available for product anti-Twist2 antibody (A04422)?
M. Li
Verified customer
Asked: 2019-07-10
Answer
We do provide the blocking peptide for product anti-Twist2 antibody (A04422). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-10
Question
Would A04422 anti-Twist2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
As indicated on the product datasheet, A04422 anti-Twist2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-07
Question
I see that the anti-Twist2 antibody A04422 works with WB, what is the protocol used to produce the result images on the product page?
G. Miller
Verified customer
Asked: 2019-04-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-11
Question
My lab would like to test anti-Twist2 antibody A04422 on rat pubmed:11062344} for research purposes, then I may be interested in using anti-Twist2 antibody A04422 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Johnson
Verified customer
Asked: 2019-03-14
Answer
The products we sell, including anti-Twist2 antibody A04422, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-14
Question
Would anti-Twist2 antibody A04422 work for WB with pubmed:11062344}?
Verified Customer
Verified customer
Asked: 2017-07-18
Answer
According to the expression profile of pubmed:11062344}, TWIST2 is highly expressed in pubmed:11062344}. So, it is likely that anti-Twist2 antibody A04422 will work for WB with pubmed:11062344}.
Boster Scientific Support
Answered: 2017-07-18
Question
We are currently using anti-Twist2 antibody A04422 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
T. Collins
Verified customer
Asked: 2017-03-08
Answer
The anti-Twist2 antibody (A04422) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-08
Question
Is this A04422 anti-Twist2 antibody reactive to the isotypes of TWIST2?
T. Yang
Verified customer
Asked: 2015-01-08
Answer
The immunogen of A04422 anti-Twist2 antibody is Peptide sequence around aa.26~30(P-K-R-F-G) derived from Human Twist2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-01-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pubmed:11062344} using anti-Twist2 antibody A04422. Let me know if you need anything else.
D. Dhar
Verified customer
Asked: 2013-05-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-05-24
Question
Do you have a BSA free version of anti-Twist2 antibody A04422 available?
C. Evans
Verified customer
Asked: 2013-04-25
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Twist2 antibody A04422 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-04-25
Question
I was wanting to use your anti-Twist2 antibody for WB for rat pubmed:11062344} on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat pubmed:11062344} identification?
D. Kulkarni
Verified customer
Asked: 2013-03-20
Answer
As indicated on the product datasheet, A04422 anti-Twist2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pubmed:11062344} in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-03-20
