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Product Info Summary
| SKU: | M01778 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TXNRD1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01778
BM5241 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TXNRD1 Rabbit Monoclonal Antibody catalog # M01778. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TXNRD1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01778)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACCE-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TXNRD1
Reactive Species
M01778 is reactive to TXNRD1 in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
70.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01778 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human Jurkat whole cell, human PC-3 whole cell, human A549 whole cell, human HepG2 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of TXNRD1 using anti-TXNRD1 antibody (M01778).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNRD1 antigen affinity purified monoclonal antibody (Catalog # M01778) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNRD1 at approximately 55 kDa. The expected band size for TXNRD1 is at 71 kDa.
Specific Publications For Anti-TXNRD1 Rabbit Monoclonal Antibody (M01778)
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5 Customer Q&As for Anti-TXNRD1 Rabbit Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01778?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of TXNRD1 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Is a blocking peptide available for product anti-TXNRD1 Monoclonal antibody (M01778)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
We do provide the blocking peptide for product anti-TXNRD1 Monoclonal antibody (M01778). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-04
Question
I see that the anti-TXNRD1 Monoclonal antibody M01778 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-04
Question
Is there a BSA free version of anti-TXNRD1 Monoclonal antibody M01778 available?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TXNRD1 Monoclonal antibody M01778 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-14
Question
We are currently using anti-TXNRD1 Monoclonal antibody M01778 for rat tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
J. Gonzalez
Verified customer
Asked: 2017-06-28
Answer
The anti-TXNRD1 Monoclonal antibody (M01778) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-28
