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Product Info Summary
|Reactive Species:||Human, Mouse, Rabbit, Rat|
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Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)
Boster Bio Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) catalog # MA1100. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1100)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Rat tyrosine hydroxylase (TH).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
MA1100 is reactive to Th in Human, Mouse, Rabbit, Rat
MA1100 is guaranteed for IHC, WB Boster Guarantee
Background of Tyrosine Hydroxylase
Tyrosine hydroxylase is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Human TH gene contains 13 primary exons and spans approximately 8 kb. TH is in the 11p15.5 region.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, rat, rabbit, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, rat, rabbit, -
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, rabbit
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Tyrosine Hydroxylase using anti-Tyrosine Hydroxylase antibody (MA1100).
Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Tyrosine Hydroxylase Antibody (MA1100) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 2. Western blot analysis of Tyrosine Hydroxylase using anti-Tyrosine Hydroxylase antibody (MA1100).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate,
Lane 3: human U-87MG cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tyrosine Hydroxylase antigen affinity purified monoclonal antibody (Catalog # MA1100) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase at approximately 59KD. The expected band size for Tyrosine Hydroxylase is at 59KD.
Gene/Protein Information For Th (Source: Uniprot.org, NCBI)
biopterin-dependent aromatic amino acid hydroxylase family
TH; DYT14; DYT5b; EC 1.14.16; EC 18.104.22.168; TH mouse; TH; TYH dystonia 14; TYH; Tyrosine 3-hydroxylase; tyrosine 3-monooxygenase; Tyrosine Hydroxylase immunohistochemistry; Tyrosine Hydroxylase mouse; Tyrosine Hydroxylase Th|The|tyrosine hydroxylase|tyrosine 3-monooxygenase|tyrosine 3-hydroxylase*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Th, check out the Th Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Th: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
MA1100 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu Y,Li L,Qiu M,Tan L,Zhang M,Li J,Zhu H,Jiang S,Su X,Li A.Renal and cerebral RAS interaction contributes to diabetic kidney disease.Am J Transl Res.2019 May 15;11(5):2925-2939.PMID:31217864;PMCID:PMC6556645.
MA1100 usage in article: APP:WB, SAMPLE: BRAIN TISSUE, DILUTION:1:200
NPPB prevents postoperative peritoneal adhesion formation by blocking volume-activated Cl - current. Changqing Zhou,Shanshan Li,Min Wang,Zhongmei Chen,Guoguang Peng
MA1100 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:150
He X,Liu Z,Pang Y,Xu W,Zhao L,Li H.Downregulation of transcription factor TCTP elevates microRNA-200a expression to restrain Myt1L expression, thereby improving neurobehavior and oxidative stress injury in cerebral palsy rats.Cell Cycle.2020 Apr;19(8):855
MA1100 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Song L,Yao L,Zhang L,Piao Z,Lu Y.Schizandrol A protects against Aβ1-42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons.Naunyn Schmiedebergs Arch Pharmacol.2020 Sep;393(9):1739-1752.doi:10.1007/s00
MA1100 usage in article: APP:WB, SAMPLE:SH-SY5Y CELLS, DILUTION:1:200
Tai S,Zheng Q,Zhai S,Cai T,Xu L,Yang L,Jiao L,Zhang C.Alpha-Lipoic Acid Mediates Clearance of Iron Accumulation by Regulating Iron Metabolism in a Parkinson‘s Disease Model Induced by 6-OHDA.Front Neurosci. 2020 Jun 25;14:612.doi:10.3389/fnins.2020.
MA1100 usage in article: APP:IHC, SAMPLE:RAT BRAIN TISSUE, DILUTION:1:100
Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2
Hu W, Ye Y, Wang J, Zhang W, Chen A, Guo F. Artif Cells Nanomed Biotechnol. 2013 Aug;41(4):249-54. Doi: 10.3109/10731199.2012.731412. Epub 2013 Jan 10. Bone Morphogenetic Proteins Induce Rabbit Bone Marrow-Derived Mesenchyme Stem Cells To Differen...
Li X, Jiang Yh, Jiang P, Yang Jl, Ma Df, Yang Ch. Chin J Integr Med. 2014 Jul;20(7):524-33. Doi: 10.1007/S11655-014-1861-Z. Epub 2014 Jun 28. Effect Of Guizhi Decoction ([Symbols; See Text]) On Heart Rate Variability And Regulation Of Cardiac Auto...
Su Y, Fu Y, Zhang H, Shi Z, Zhang J, Gao L. Fish Physiol Biochem. 2015 Oct;41(5):1093-104. Doi: 10.1007/S10695-015-0071-8. Epub 2015 Jun 3. Identification And Expression Of Srf Targeted By Mir-133A During Early Development Of Paralichthys Olivaceus.
Deng Y, Jiao C, Mi C, Xu B, Li Y, Wang F, Liu W, Xu Z. Mol Neurobiol. 2015 Feb;51(1):68-88. Doi: 10.1007/S12035-014-8789-3. Epub 2014 Jun 28. Melatonin Inhibits Manganese-Induced Motor Dysfunction And Neuronal Loss In Mice: Involvement Of Oxidativ...
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