|Product Name||Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Tyrosine Hydroxylase, tyrosine hydroxylase (TH) detection. Tested with WB, IHC-P, IHC-F in Human;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1100)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Rat tyrosine hydroxylase(TH).|
|Reactivity||Human, Rabbit, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, rat, rabbit, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, rat, rabbit, -
Western blot, 0.25-0.5μg/ml, Human, rat, rabbit
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
Images And Assay Conditions
Figure 1. IHC analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MA1100).
Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti- Tyrosine Hydroxylase Antibody (MA1100) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Figure 2. Western blot analysis of Tyrosine Hydroxylase using anti-Tyrosine Hydroxylase antibody (MA1100).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate,
Lane 3: human U-87MG cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tyrosine Hydroxylase antigen affinity purified monoclonal antibody (Catalog # MA1100) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase at approximately 59KD. The expected band size for Tyrosine Hydroxylase is at 59KD.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Tyrosine 3-monooxygenase|
|Tissue Specificity||TH: Mainly expressed in the brain and adrenalglands.|
|Alternative Names||Tyrosine 3-monooxygenase;126.96.36.199;Tyrosine 3-hydroxylase;TH;Th;|
|Molecular Weight||55966 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Plays an important role in the physiology of adrenergic neurons.|
|Research Areas||Cancer, Cancer Metabolism, Cell Type Marker, Endocrine Metabolism, Hormone Biosynthesis, Hypoxia, Metabolic Signaling Pathway, Metabolism, Metabolism Processes, Neuron Marker, Neuroscience, Neurotransmitter, Pathways And Processes, Response To Hypoxia
*You can search these to find other products in these research areas.
|Background||Tyrosine hydroxylase is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Human TH gene contains 13 primary exons and spans approximately 8 kb. TH is in the 11p15.5 region.|
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