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TH antibody (PB9449) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 31 Publications

pb9449 th primary antibodies if testing 4_1

IF analysis of Tyrosine Hydroxylase/TH using anti-Tyrosine Hydroxylase/TH antibody (PB9449).
Tyrosine Hydroxylase/TH was detected in a paraffin-embedded section of mouse brian tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Tyrosine Hydroxylase/TH Antibody (PB9449) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-Tyrosine Hydroxylase/TH Antibody Picoband®

Name: Anti-Tyrosine Hydroxylase/TH Antibody Picoband®
Brand: Boster Bio
SKU: PB9449
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

TH antibody

31 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IF, IHC, WB

Tested Applications

TH antibody was positively detected by WB in:

rat brain tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

TH antibody was positively detected by IHC in:

human brain tissue, mouse brain tissue, rat brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

TH antibody was positively detected by IF in:

mouse brian tissue, rat brian tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Rat

Tested

Mouse

Tested

Human

Tested

Recommend

Monkey

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Product Information

Product Name

Anti-Tyrosine Hydroxylase/TH Antibody Picoband®
View all TH Antibodies

Form

Lyophilized

Uniprot

P07101

Gene ID

7054

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Tyrosine Hydroxylase, identical to the related mouse and rat sequences.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

59 kDa

Calculated Molecular Weight

58.6 kDa

Background of TH

TH is equal to tyrosine hydroxylase. The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. In humans, tyrosine hydroxylase is encoded by the TH gene, and the enzyme is present in the central nervous system (CNS), peripheral sympathetic neurons and the adrenal medulla. Tyrosine hydroxylase, phenylalanine hydroxylase and tryptophan hydroxylase together make up the family of aromatic amino acid hydroxylases (AAAHs).

Cite This Product

Anti-Tyrosine Hydroxylase/TH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9449)

Protocols

Western Blot protocol for TH antibody

Review Protocol

Immunohistochemistry (IHC) protocol for TH antibody

Review Protocol

Immunofluorescence (IF) protocol for TH antibody

Review Protocol

Publications

The novel GLP-1/GIP dual receptor agonist DA5-CH is superior to tirzepatide and exendin-4 in the 6-OHDA Parkinson rat model

Published: 2026-04-17

Journal: Frontiers in Endocrinology

Application: IHC

Impact Factor: 4.60

Species: Rat

PMID: 42165019

SKU	PB9449
Application	Immunohistochemistry
Sample	Normal mouse brain and Alzheimer’s model mouse brain tissue
Sample Processing Description	Paraffin-embedded normal mouse brain and Alzheimer’s model mouse brain.
Other Reagents	Goat serum, DAB
Primary Antibody	Tyrosine Hydroxylase/TH Antibody Picoband®
Primary Incubation	1:500, overnight at 4 ℃
Secondary Antibody	Two-step IHC kit
Secondary Incubation	37 minutes in 37 ℃
Detection	Imaging system:Leica DM2500
Results Summary	TH is a marker of catecholaminergic neurons, specifically labeling dopaminergic, noradrenergic, and adrenergic neurons. IHC results showed that TH expression in the medulla was markedly lower in Alzheimer’s mouse compared to normal mouse.

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Anti-Tyrosine Hydroxylase/TH Antibody Picoband®

TH antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

The novel GLP-1/GIP dual receptor agonist DA5-CH is superior to tirzepatide and exendin-4 in the 6-OHDA Parkinson rat model

Hydrogen sulfide protects dopaminergic neurons via NLRP3 inflammasome inhibition in an MPTP mouse model of Parkinson’s disease

Oligodendrocyte-Specific STAT5B Overexpression Ameliorates Myelin Impairment in Experimental Models of Parkinson’s Disease

Nociceptor neurons promote PDAC progression and cancer pain by interaction with cancer-associated fibroblasts and suppression of natural killer cells

Central Angiotensin II type 1 receptor deficiency alleviates renal fibrosis by reducing sympathetic nerve discharge in nephrotoxic folic acid–induced chronic kidney disease

LncTCONS_00058568 is involved in the pathophysiologic processes mediated by P2X7R in the lower thoracic spinal cord after acute kidney injury

Folic Acid Rescues Dopaminergic Neurons in MPTP-Induced Mice by Inhibiting the NLRP3 Inflammasome and Ameliorating Mitochondrial Impairment

Exposure of embryos to fenbuconazole causes persistent neurotoxicity in adult zebrafish

Neural and central mechanisms of kidney fibrosis after relief of ureteral obstruction.

Ticagrelor Can Regulate the Ion Channel Characteristics of Superior Cervical Ganglion Neurons after Myocardial Infarction

Neural stem cell secretome exerts a protective effect on damaged neuron mitochondria in Parkinson's disease model

Moderate ethanol drinking is sufficient to alter Ventral Tegmental Area dopamine neurons activity via functional and structural remodeling of GABAergic transmission

PAC1 Receptor Mediates Electroacupuncture-Induced Neuro and Immune Protection During Cisplatin Chemotherapy

Age-Related Cognitive and Motor Decline in a Mouse Model of CDKL5 Deficiency Disorder is Associated with Increased Neuronal Senescence and Death

lncRNA NEAT1 prompts autophagy and apoptosis in MPTP-induced Parkinson’s disease by impairing miR-374c-5p

Harpagide Inhibits Microglial Activation and Protects Dopaminergic Neurons as Revealed by Nanoelectrode Amperometry†

TRIM3 attenuates apoptosis in Parkinson's disease via activating PI3K/AKT signal pathway

Alpha-Lipoic Acid Mediates Clearance of Iron Accumulation by Regulating Iron Metabolism in a Parkinson’s Disease Model Induced by 6-OHDA

Downregulation of transcription factor TCTP elevates microRNA-200a expression to restrain Myt1L expression, thereby improving neurobehavior and oxidative stress injury in cerebral palsy rats

Renal and cerebral RAS interaction contributes to diabetic kidney disease

Neuroprotective effect of arctigenin against neuroinflammation and oxidative stress induced by rotenone

Effect of the changes of NMDA receptor in hypothalamic paraventricular nucleus on cardiac function and sympathetic nervous activity in rats with heart failure

Graphene oxide nanosheets at trace concentrations elicit neurotoxicity in the offspring of zebrafish

Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2

Ultra-trace graphene oxide in a water environment triggers Parkinson's disease-like symptoms and metabolic disturbance in zebrafish larvae

Botanical Drug Puerarin Coordinates with Nerve Growth Factor in the Regulation of Neuronal Survival and Neuritogenesis via Activating ERK1/2 and PI3K/Akt Signaling Pathways in the Neurite Extension Process

Puerarin attenuates neuronal degeneration and blocks oxidative stress to elicit a neuroprotective effect on substantia nigra injury in 6-OHDA-lesioned rats

Derivation of multipotent nestin+/CD271−/STRO-1− mesenchymal-like precursors from human embryonic stem cells in chemically defined conditions

Effect of adenovirus-mediated brain derived neurotrophic factor in early retinal neuropathy of diabetes in rats

Cholinergic neuronal differentiation of bone marrow mesenchymal stem cells in rhesus monkeys

Induced Multilineage Differentiation of Chicken Embryonic Germ Cells via Embryoid Body Formation

Review

Have you used Anti-Tyrosine Hydroxylase/TH Antibody Picoband®?

1 Review For Anti-Tyrosine Hydroxylase/TH Antibody Picoband®

IHC using Tyrosine Hydroxylase/TH Antibody Picoband® (P00683) showed clear staining with no background, revealing markedly reduced TH expression in the medulla of Alzheimer’s mouse brain compared to normal mouse brain.

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