radio
SKU MA1100
Size 100μg/vial
Reactivity Human, Rabbit, Rat
Clonality Monoclonal
Clone Number TH-16
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)
SKU/Catalog Number MA1100
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Tyrosine Hydroxylase, tyrosine hydroxylase (TH) detection. Tested with WB, IHC-P, IHC-F in Human;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1100)
Host Mouse
Immunogen Rat tyrosine hydroxylase(TH).
Reactivity Human, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, rat, rabbit, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, rat, rabbit, -
Western blot, 0.25-0.5μg/ml, Human, rat, rabbit

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1100 1 IHC anti tyrosine hydroxylase antibody monoclonal th 16.jpg

Figure 1. IHC analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MA1100).
Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti- Tyrosine Hydroxylase Antibody (MA1100) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

/antibody/ma1100 2 WB anti tyrosine hydroxylase antibody monoclonal th 16.jpg

Figure 2. Western blot analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MA1100).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Tyrosine Hydroxylase antigen affinity purified monoclonal antibody (Catalog # MA1100) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase at approximately 59KD. The expected band size for Tyrosine Hydroxylase is at 59KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P04177
Gene Name Th
Protein Name Tyrosine 3-monooxygenase
Tissue Specificity TH: Mainly expressed in the brain and adrenalglands.
Alternative Names Tyrosine 3-monooxygenase;1.14.16.2;Tyrosine 3-hydroxylase;TH;Th;
Molecular Weight 55966 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Plays an important role in the physiology of adrenergic neurons.
Background Tyrosine hydroxylase is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Human TH gene contains 13 primary exons and spans approximately 8 kb. TH is in the 11p15.5 region.

Order Product (MA1100)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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We promise all of our products perform as described in datasheets.

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Publications

Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2
S100+cells: A new neuro-immune cross-talkers in lymph organs
Effect of adenovirus-mediated brain derived neurotrophic factor in early retinal neuropathy of diabetes in rats
Hu W, Ye Y, Wang J, Zhang W, Chen A, Guo F. Artif Cells Nanomed Biotechnol. 2013 Aug;41(4):249-54. Doi: 10.3109/10731199.2012.731412. Epub 2013 Jan 10. Bone Morphogenetic Proteins Induce Rabbit Bone Marrow-Derived Mesenchyme Stem Cells To Differen...
Li X, Jiang Yh, Jiang P, Yang Jl, Ma Df, Yang Ch. Chin J Integr Med. 2014 Jul;20(7):524-33. Doi: 10.1007/S11655-014-1861-Z. Epub 2014 Jun 28. Effect Of Guizhi Decoction ([Symbols; See Text]) On Heart Rate Variability And Regulation Of Cardiac Auto...
Su Y, Fu Y, Zhang H, Shi Z, Zhang J, Gao L. Fish Physiol Biochem. 2015 Oct;41(5):1093-104. Doi: 10.1007/S10695-015-0071-8. Epub 2015 Jun 3. Identification And Expression Of Srf Targeted By Mir-133A During Early Development Of Paralichthys Olivaceus.
Deng Y, Jiao C, Mi C, Xu B, Li Y, Wang F, Liu W, Xu Z. Mol Neurobiol. 2015 Feb;51(1):68-88. Doi: 10.1007/S12035-014-8789-3. Epub 2014 Jun 28. Melatonin Inhibits Manganese-Induced Motor Dysfunction And Neuronal Loss In Mice: Involvement Of Oxidativ...
Wu L, Zhao Q, Zhu X, Peng M, Jia C, Wu W, Zheng J, Wu Xz. Brain Pathol. 2010 Nov;20(6):1042-54. Doi: 10.1111/J.1750-3639.2010.00410.X. Epub 2010 Jun 14. A Novel Function Of Microrna Let-7D In Regulation Of Galectin-3 Expression In Attention Defici...
Wan C, Liu Nn, Liu Lm, Cai N, Chen L. Int J Ophthalmol. 2010;3(2):145-8. Doi: 10.3980/J.Issn.2222-3959.2010.02.12. Epub 2010 Jun 18. Effect Of Adenovirus-Mediated Brain Derived Neurotrophic Factor In Early Retinal Neuropathy Of Diabetes In Rats.
Zhao J, Cheng Yy, Fan W, Yang Cb, Ye Sf, Cui W, Wei W, Lao Lx, Cai J, Han Yf, Rong Jh. Cns Neurosci Ther. 2015 Jan;21(1):61-70. Doi: 10.1111/Cns.12334. Epub 2014 Oct 14. Botanical Drug Puerarin Coordinates With Nerve Growth Factor In The Regulatio...
Wu Y, Ge C, Zeng W, Zhang C. Stem Cells Dev. 2010 Feb;19(2):195-202. Doi: 10.1089/Scd.2008.0383. Induced Multilineage Differentiation Of Chicken Embryonic Germ Cells Via Embryoid Body Formation.
Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tyrosine hydroxylase antibody, th antibody