|Validated Species:||Human, Rabbit, Rat|
Data & Images
|Product Name||Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)|
|Description||Mouse IgG monoclonal antibody for Tyrosine Hydroxylase, tyrosine hydroxylase (TH) detection. Tested with WB, IHC-P, IHC-F in Human;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) TH-16 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1100)|
|Replacement Item||This antibody may replace the following items: sc-47708|sc-7848|sc-25269|sc-7847|sc-374048|sc-374047|sc-73151|sc-73152|sc-14007|sc-136100 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rabbit, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Rat tyrosine hydroxylase(TH).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tyrosine 3-monooxygenase|
|Molecular Weight||55966 MW|
|Protein Function||Plays an important role in the physiology of adrenergic neurons.|
|Tissue Specificity||TH: Mainly expressed in the brain and adrenalglands.|
|Sequence Similarities||Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.|
|Alternative Names||Tyrosine 3-monooxygenase;220.127.116.11;Tyrosine 3-hydroxylase;TH;Th;|
Background for Tyrosine 3-monooxygenase
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, rat, rabbit, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, rat, rabbit, -
Western blot, 0.25-0.5μg/ml, Human, rat, rabbit
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) Images
Click the images to enlarge.
Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti- Tyrosine Hydroxylase Antibody (MA1100) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Tyrosine Hydroxylase antigen affinity purified monoclonal antibody (Catalog # MA1100) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase at approximately 59KD. The expected band size for Tyrosine Hydroxylase is at 59KD.