Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)
|Reactivity||Human, Rabbit, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Tyrosine Hydroxylase, tyrosine hydroxylase (TH) detection. Tested with WB, IHC-P, IHC-F in Human;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1100)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Rat tyrosine hydroxylase(TH).|
|Reactivity||Human, Rabbit, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, rat, rabbit, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, rat, rabbit, -
Western blot, 0.25-0.5μg/ml, Human, rat, rabbit
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
Images And Assay Conditions
Figure 1. IHC analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MA1100).
Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml mouse anti- Tyrosine Hydroxylase Antibody (MA1100) overnight at 4Â°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Figure 2. Western blot analysis of Tyrosine Hydroxylase using anti-Tyrosine Hydroxylase antibody (MA1100).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate,
Lane 3: human U-87MG cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tyrosine Hydroxylase antigen affinity purified monoclonal antibody (Catalog # MA1100) at 0.5 Î¼g/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase at approximately 59KD. The expected band size for Tyrosine Hydroxylase is at 59KD.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Tyrosine 3-monooxygenase|
|Tissue Specificity||TH: Mainly expressed in the brain and adrenalglands.|
|Alternative Names||Tyrosine 3-monooxygenase;22.214.171.124;Tyrosine 3-hydroxylase;TH;Th;|
|Molecular Weight||55966 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Plays an important role in the physiology of adrenergic neurons.|
|Research Areas||Human, Rabbit, Rat
*You can search these to find other products in these research areas.
|Background||Tyrosine hydroxylase is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Human TH gene contains 13 primary exons and spans approximately 8 kb. TH is in the 11p15.5 region.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact firstname.lastname@example.org
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to email@example.com.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to tyrosine hydroxylase antibody, th antibody