Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)

Boster Bio Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) catalog # MA1100. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat. Cited in 15 publication(s).

Product Info Summary

SKU: MA1100
Size: 100μg/vial
Reactive Species: Human, Mouse, Rabbit, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)

See all Tyrosine Hydroxylase products

SKU/Catalog Number

MA1100

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) catalog # MA1100. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1100)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

TH-16

Isotype

Mouse IgG1

Immunogen

Rat tyrosine hydroxylase (TH).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1100 is reactive to Th in Human, Mouse, Rabbit, Rat

Applications

MA1100 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, rat, rabbit, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, rat, rabbit, -
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, rabbit

Validation Images & Assay Conditions

Gene/Protein Information For Th (Source: Uniprot.Org, NCBI)

Uniprot ID

P04177

Gene ID

25085

Gene Name

Th

Full Name

Tyrosine 3-monooxygenase

Weight

55.966kDa

Superfamily

biopterin-dependent aromatic amino acid hydroxylase family

Alternative Names

TH; DYT14; DYT5b; EC 1.14.16; EC 1.14.16.2; TH mouse; TH; TYH dystonia 14; TYH; Tyrosine 3-hydroxylase; tyrosine 3-monooxygenase; Tyrosine Hydroxylase immunohistochemistry; Tyrosine Hydroxylase mouse; Tyrosine Hydroxylase Th|The|tyrosine hydroxylase|tyrosine 3-monooxygenase|tyrosine 3-hydroxylase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Th, check out the Th Infographic

Th infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Th: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1100 has been cited in 15 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu Y,Li L,Qiu M,Tan L,Zhang M,Li J,Zhu H,Jiang S,Su X,Li A.Renal and cerebral RAS interaction contributes to diabetic kidney disease.Am J Transl Res.2019 May 15;11(5):2925-2939.PMID:31217864;PMCID:PMC6556645.
Species: Rat
MA1100 usage in article: APP:WB, SAMPLE: BRAIN TISSUE, DILUTION:1:200

NPPB prevents postoperative peritoneal adhesion formation by blocking volume-activated Cl - current. Changqing Zhou,Shanshan Li,Min Wang,Zhongmei Chen,Guoguang Peng
Species: Mouse
MA1100 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:150

He X,Liu Z,Pang Y,Xu W,Zhao L,Li H.Downregulation of transcription factor TCTP elevates microRNA-200a expression to restrain Myt1L expression, thereby improving neurobehavior and oxidative stress injury in cerebral palsy rats.Cell Cycle.2020 Apr;19(8):855
Species: Rat
MA1100 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Song L,Yao L,Zhang L,Piao Z,Lu Y.Schizandrol A protects against Aβ1-42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons.Naunyn Schmiedebergs Arch Pharmacol.2020 Sep;393(9):1739-1752.doi:10.1007/s00
Species: Human
MA1100 usage in article: APP:WB, SAMPLE:SH-SY5Y CELLS, DILUTION:1:200

Tai S,Zheng Q,Zhai S,Cai T,Xu L,Yang L,Jiao L,Zhang C.Alpha-Lipoic Acid Mediates Clearance of Iron Accumulation by Regulating Iron Metabolism in a Parkinson‘s Disease Model Induced by 6-OHDA.Front Neurosci. 2020 Jun 25;14:612.doi:10.3389/fnins.2020.
Species: Rat
MA1100 usage in article: APP:IHC, SAMPLE:RAT BRAIN TISSUE, DILUTION:1:100

Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2

Hu W, Ye Y, Wang J, Zhang W, Chen A, Guo F. Artif Cells Nanomed Biotechnol. 2013 Aug;41(4):249-54. Doi: 10.3109/10731199.2012.731412. Epub 2013 Jan 10. Bone Morphogenetic Proteins Induce Rabbit Bone Marrow-Derived Mesenchyme Stem Cells To Differen...

Li X, Jiang Yh, Jiang P, Yang Jl, Ma Df, Yang Ch. Chin J Integr Med. 2014 Jul;20(7):524-33. Doi: 10.1007/S11655-014-1861-Z. Epub 2014 Jun 28. Effect Of Guizhi Decoction ([Symbols; See Text]) On Heart Rate Variability And Regulation Of Cardiac Auto...

Su Y, Fu Y, Zhang H, Shi Z, Zhang J, Gao L. Fish Physiol Biochem. 2015 Oct;41(5):1093-104. Doi: 10.1007/S10695-015-0071-8. Epub 2015 Jun 3. Identification And Expression Of Srf Targeted By Mir-133A During Early Development Of Paralichthys Olivaceus.

Deng Y, Jiao C, Mi C, Xu B, Li Y, Wang F, Liu W, Xu Z. Mol Neurobiol. 2015 Feb;51(1):68-88. Doi: 10.1007/S12035-014-8789-3. Epub 2014 Jun 28. Melatonin Inhibits Manganese-Induced Motor Dysfunction And Neuronal Loss In Mice: Involvement Of Oxidativ...

Wu L, Zhao Q, Zhu X, Peng M, Jia C, Wu W, Zheng J, Wu Xz. Brain Pathol. 2010 Nov;20(6):1042-54. Doi: 10.1111/J.1750-3639.2010.00410.X. Epub 2010 Jun 14. A Novel Function Of Microrna Let-7D In Regulation Of Galectin-3 Expression In Attention Defici...

Wan C, Liu Nn, Liu Lm, Cai N, Chen L. Int J Ophthalmol. 2010;3(2):145-8. Doi: 10.3980/J.Issn.2222-3959.2010.02.12. Epub 2010 Jun 18. Effect Of Adenovirus-Mediated Brain Derived Neurotrophic Factor In Early Retinal Neuropathy Of Diabetes In Rats.

Zhao J, Cheng Yy, Fan W, Yang Cb, Ye Sf, Cui W, Wei W, Lao Lx, Cai J, Han Yf, Rong Jh. Cns Neurosci Ther. 2015 Jan;21(1):61-70. Doi: 10.1111/Cns.12334. Epub 2014 Oct 14. Botanical Drug Puerarin Coordinates With Nerve Growth Factor In The Regulatio...

Wu Y, Ge C, Zeng W, Zhang C. Stem Cells Dev. 2010 Feb;19(2):195-202. Doi: 10.1089/Scd.2008.0383. Induced Multilineage Differentiation Of Chicken Embryonic Germ Cells Via Embryoid Body Formation.

Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...

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13 Customer Q&As for Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)

Question

I would like to test anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100 on rabbit substantia nigra for research purposes, then I may be interested in using anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

The products we sell, including anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-13

Question

Is this MA1100 anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) reactive to the isotypes of TH?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

The immunogen of MA1100 anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) is Rat tyrosine hydroxylase(TH). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-29

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for substantia nigra using anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-11

Question

I see that the anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-10

Question

Is a blocking peptide available for product anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) (MA1100)?

Verified Customer

Verified customer

Asked: 2019-05-08

Answer

We do provide the blocking peptide for product anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) (MA1100). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-08

Question

I have attached the WB image, lot number and protocol we used for substantia nigra using anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100. Please let me know if you require anything else.

D. Wu

Verified customer

Asked: 2019-04-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-02

Question

Would MA1100 anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

W. Jackson

Verified customer

Asked: 2019-03-05

Answer

It shows on the product datasheet, MA1100 anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-03-05

Question

Can you help my question with product MA1100, anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-09-28

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1100 anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-28

Question

Do you have a BSA free version of anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100 available?

Verified Customer

Verified customer

Asked: 2018-01-10

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-01-10

Question

Our lab want to know about using your anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) for cellular response to manganese ion studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-10-18

Answer

Thank you for your inquiry. This MA1100 anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) is validated on rat brain tissue, tissue lysate, mouse brain. It is guaranteed to work for IHC, WB in human, rabbit, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-10-18

Question

We are currently using anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rabbit, rat. Is it likely that the antibody can work on bovine tissues as well?

L. Krishna

Verified customer

Asked: 2016-09-28

Answer

The anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) (MA1100) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-09-28

Question

I was wanting to use your anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) for WB for rabbit substantia nigra on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rabbit substantia nigra identification?

B. Zhang

Verified customer

Asked: 2016-02-25

Answer

You can see on the product datasheet, MA1100 anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) has been validated for IHC, WB on human, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in rabbit substantia nigra in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-02-25

Question

Will anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100 work for WB with substantia nigra?

K. Anderson

Verified customer

Asked: 2015-06-05

Answer

According to the expression profile of substantia nigra, TH is highly expressed in substantia nigra. So, it is likely that anti-Tyrosine Hydroxylase antibody (Monoclonal, TH-16) MA1100 will work for WB with substantia nigra.

Boster Scientific Support

Answered: 2015-06-05

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