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Product Info Summary
| SKU: | M01917-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody
SKU/Catalog Number
M01917-1
BM4394 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody catalog # M01917-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C long term. Avoid freeze / thaw cycle.
Cite This Product
Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01917-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ECA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Tyrosine Hydroxylase
Reactive Species
M01917-1 is reactive to TH in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
58.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01917-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
IHC: human kidney tissue
ICC/IF: PC-12 cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Tyrosine Hydroxylase expression in PC-3 cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Tyrosine Hydroxylase Antibody .
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Immunofluorescent analysis of PC-12 cells, using Tyrosine Hydroxylase Antibody.
Specific Publications For Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody (M01917-1)
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody
Question
See below the WB image, lot number and protocol we used for substantia nigra using anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-02
Question
I see that the anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-24
Question
Does M01917-1 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
As indicated on the product datasheet, M01917-1 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for substantia nigra using anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-15
Question
Is a blocking peptide available for product anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody (M01917-1)?
L. Huang
Verified customer
Asked: 2019-04-11
Answer
We do provide the blocking peptide for product anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody (M01917-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-04-11
Question
Is this M01917-1 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody reactive to the isotypes of TH?
T. Krishna
Verified customer
Asked: 2018-04-16
Answer
The immunogen of M01917-1 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody is A synthesized peptide derived from human Tyrosine Hydroxylase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-16
Question
Do you have a BSA free version of anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1 available?
N. Parker
Verified customer
Asked: 2017-11-29
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-29
Question
Can you help my question with product M01917-1, anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Jha
Verified customer
Asked: 2017-11-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01917-1 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-21
Question
Will anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1 work for WB with substantia nigra?
Verified Customer
Verified customer
Asked: 2017-06-13
Answer
According to the expression profile of substantia nigra, TH is highly expressed in substantia nigra. So, it is likely that anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1 will work for WB with substantia nigra.
Boster Scientific Support
Answered: 2017-06-13
Question
We want to test anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1 on human substantia nigra for research purposes, then I may be interested in using anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Johnson
Verified customer
Asked: 2016-05-25
Answer
The products we sell, including anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-05-25
Question
I was wanting to use your anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody for WB for human substantia nigra on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human substantia nigra identification?
B. Baker
Verified customer
Asked: 2015-03-04
Answer
As indicated on the product datasheet, M01917-1 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human substantia nigra in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-04
Question
We are currently using anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917-1 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
P. Anderson
Verified customer
Asked: 2014-01-15
Answer
The anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody (M01917-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-01-15
