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Product Info Summary
| SKU: | M01917 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody
SKU/Catalog Number
M01917
BM4568 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody catalog # M01917. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01917)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GOO-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Tyrosine Hydroxylase
Reactive Species
M01917 is reactive to TH in Human, Mouse, Rat
Observed Molecular Weight
59 kDa
Calculated molecular weight
58.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01917 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
IHC: human brain tissue
Validation Images & Assay Conditions
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Western blot analysis of TH using anti-TH antibody (M01917).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TH antigen affinity purified monoclonal antibody (M01917) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TH at approximately 59 kDa. The expected band size for TH is at 59 kDa.
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Immunohistochemical analysis of paraffin-embedded human brain, using Tyrosine Hydroxylase Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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IF analysis of TH using anti-TH antibody (M01917).
TH was detected in an immunocytochemical section of mouse brain tissue. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1:200 rabbit anti-TH Antibody (M01917) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody (M01917)
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Customer Reviews
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1 Reviews For Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody
The Anti-TH antibody (M01917) demonstrated excellent specificity and clear fluorescence staining in IF analysis of paraffin-embedded mouse striatum sections, accurately labeling dopaminergic neurons with minimal background.
Excellent
| SKU | M01917 |
|---|---|
| Application | Immunofluorescence |
| Sample | mouse brain |
| Sample Processing Description | Sagittal sections of mouse striatum were fixed in formaldehyde for 48 hours and paraffin-embedded. |
| Other Reagents | Goat serum，DAPI，Anti-fade mounting medium |
| Primary Antibody | Iba1 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Secondary Antibody | 1:500, Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated |
| Secondary Incubation | 45 min at 37℃ |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | TH is a marker of catecholaminergic neurons and can specifically label and identify dopaminergic, noradrenergic, and adrenergic neurons. In this experiment, TH immunofluorescence was performed to observe the distribution of dopaminergic neurons in the striatum. The results show clear staining and precise localization. |
Zengting Li, Huazhong University of Science and Technology
Verified customer
Submitted 2026-02-12
Customer Q&As
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12 Customer Q&As for Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody
Question
Will anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917 work for IF with substantia nigra?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
According to the expression profile of substantia nigra, TH is highly expressed in substantia nigra. So, it is likely that anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917 will work for IF with substantia nigra.
Boster Scientific Support
Answered: 2020-03-12
Question
I was wanting to use your anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody for IF for rat substantia nigra on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat substantia nigra identification?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
It shows on the product datasheet, M01917 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody has been tested for Flow Cytometry, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat substantia nigra in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-10
Question
Is a blocking peptide available for product anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody (M01917)?
T. Zhao
Verified customer
Asked: 2019-07-22
Answer
We do provide the blocking peptide for product anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody (M01917). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-22
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for substantia nigra using anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-08
Question
We are currently using anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917 for mouse tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-24
Answer
The anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody (M01917) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-24
Question
Can you help my question with product M01917, anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01917 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-22
Question
Is there a BSA free version of anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917 available?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-30
Question
See attached the WB image, lot number and protocol we used for substantia nigra using anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917. Please let me know if you require anything else.
M. Mitchell
Verified customer
Asked: 2017-07-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-11
Question
I see that the anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917 works with IF, what is the protocol used to produce the result images on the product page?
S. Kulkarni
Verified customer
Asked: 2017-04-11
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-04-11
Question
Is this M01917 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody reactive to the isotypes of TH?
R. Krishna
Verified customer
Asked: 2016-05-11
Answer
The immunogen of M01917 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody is A synthesized peptide derived from human Tyrosine Hydroxylase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-05-11
Question
I am interested in to test anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917 on rat substantia nigra for research purposes, then I may be interested in using anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Jackson
Verified customer
Asked: 2016-05-03
Answer
The products we sell, including anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody M01917, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-05-03
Question
Does M01917 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Carter
Verified customer
Asked: 2013-11-04
Answer
As indicated on the product datasheet, M01917 anti-Tyrosine Hydroxylase Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-11-04
