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Product Info Summary
| SKU: | A11625 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-UBCE7IP4 RNF144A Antibody
SKU/Catalog Number
A11625
Size
100μl
Form
Liquid
Description
Boster Bio Anti-UBCE7IP4 RNF144A Antibody catalog # A11625. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBCE7IP4 RNF144A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11625)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RNF144A. AA range:151-200
Reactive Species
A11625 is reactive to RNF144A in Human, Mouse
Calculated molecular weight
32.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11625 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using UBCE7IP4 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysates from COLO cells, using RNF144A Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-UBCE7IP4 RNF144A Antibody (A11625)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-UBCE7IP4 RNF144A Antibody
Question
I was wanting to use to test anti-UBCE7IP4 antibody A11625 on mouse ovary for research purposes, then I may be interested in using anti-UBCE7IP4 antibody A11625 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Thomas
Verified customer
Asked: 2019-08-02
Answer
The products we sell, including anti-UBCE7IP4 antibody A11625, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-02
Question
I was wanting to use your anti-UBCE7IP4 antibody for WB for mouse ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse ovary identification?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
It shows on the product datasheet, A11625 anti-UBCE7IP4 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-04
Question
Would anti-UBCE7IP4 antibody A11625 work on zebrafish WB with ovary?
Verified Customer
Verified customer
Asked: 2018-10-24
Answer
Our lab technicians have not validated anti-UBCE7IP4 antibody A11625 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-UBCE7IP4 antibody A11625 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-24
Question
Would anti-UBCE7IP4 antibody A11625 work for WB with ovary?
J. Moore
Verified customer
Asked: 2016-12-02
Answer
According to the expression profile of ovary, RNF144A is highly expressed in ovary. So, it is likely that anti-UBCE7IP4 antibody A11625 will work for WB with ovary.
Boster Scientific Support
Answered: 2016-12-02
Question
Is there a BSA free version of anti-UBCE7IP4 antibody A11625 available?
S. Roberts
Verified customer
Asked: 2015-08-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-UBCE7IP4 antibody A11625 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-08-18
Question
Can you help my question with product A11625, anti-UBCE7IP4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Wu
Verified customer
Asked: 2013-08-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11625 anti-UBCE7IP4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-08-08
