Anti-UBE2D2/Ubch5B Antibody

Boster Bio Anti-UBE2D2/Ubch5B Antibody catalog # A03971-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A03971-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-UBE2D2/Ubch5B Antibody

See all UbcH5b/UBE2D2 products

SKU/Catalog Number

A03971-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-UBE2D2/Ubch5B Antibody catalog # A03971-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-UBE2D2/Ubch5B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03971-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human UBE2D2. at AA rangle: 70-150

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03971-1 is reactive to UBE2D2 in Human, Mouse, Rat

Applications

A03971-1 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For UBE2D2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P62837

Gene ID

7322

Gene Name

UBE2D2

Full Name

Ubiquitin-conjugating enzyme E2 D2

Weight

16.735kDa

Superfamily

ubiquitin-conjugating enzyme family

Alternative Names

E2(17)KB2; EC 6.3.2.19; PUBC1; UBC4/5; UBC4ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); UBC5B; UBCH4; UbcH5b; UBE2D2; Ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2 D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D2 UBE2D2 E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B ubiquitin conjugating enzyme E2 D2 ubiquitin-conjugating enzyme E2 D2|(E3-independent) E2 ubiquitin-conjugating enzyme D2|E2 ubiquitin-conjugating enzyme D2|p53-regulated ubiquitin-conjugating enzyme 1|ubiquitin carrier protein D2|ubiquitin conjugating enzyme E2D 2|ubiquitin-conjugating enzyme E2-17 kDa 2|ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)|ubiquitin-protein ligase D2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on UBE2D2, check out the UBE2D2 Infographic

UBE2D2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for UBE2D2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-UBE2D2/Ubch5B Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for teratocarcinoma using anti-UBE2D2/Ubch5B antibody A03971-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-05-04

Question

We are currently using anti-UBE2D2/Ubch5B antibody A03971-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

The anti-UBE2D2/Ubch5B antibody (A03971-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-01

Question

Will anti-UBE2D2/Ubch5B antibody A03971-1 work on goat WB with testis?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

Our lab technicians have not validated anti-UBE2D2/Ubch5B antibody A03971-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-UBE2D2/Ubch5B antibody A03971-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat testis in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-31

Question

Can you help my question with product A03971-1, anti-UBE2D2/Ubch5B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Krishna

Verified customer

Asked: 2014-12-10

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03971-1 anti-UBE2D2/Ubch5B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-12-10

Question

I see that the anti-UBE2D2/Ubch5B antibody A03971-1 works with WB, what is the protocol used to produce the result images on the product page?

A. Parker

Verified customer

Asked: 2013-05-30

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-05-30

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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A03971-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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