Click image to see more details
Product Info Summary
| SKU: | A03971-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-UBE2D2/Ubch5B Antibody
SKU/Catalog Number
A03971-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-UBE2D2/Ubch5B Antibody catalog # A03971-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE2D2/Ubch5B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03971-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human UBE2D2. AA range:98-147
Reactive Species
A03971-1 is reactive to UBE2D2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
16.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03971-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using UBE2D2 Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000
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Western blot analysis of the lysates from K562 cells using UBE2D2 antibody.
Specific Publications For Anti-UBE2D2/Ubch5B Antibody (A03971-1)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-UBE2D2/Ubch5B Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for teratocarcinoma using anti-UBE2D2/Ubch5B antibody A03971-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-04
Question
We are currently using anti-UBE2D2/Ubch5B antibody A03971-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
The anti-UBE2D2/Ubch5B antibody (A03971-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-01
Question
Will anti-UBE2D2/Ubch5B antibody A03971-1 work on goat WB with testis?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Our lab technicians have not validated anti-UBE2D2/Ubch5B antibody A03971-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-UBE2D2/Ubch5B antibody A03971-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-31
Question
Can you help my question with product A03971-1, anti-UBE2D2/Ubch5B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Krishna
Verified customer
Asked: 2014-12-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03971-1 anti-UBE2D2/Ubch5B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-12-10
Question
I see that the anti-UBE2D2/Ubch5B antibody A03971-1 works with WB, what is the protocol used to produce the result images on the product page?
A. Parker
Verified customer
Asked: 2013-05-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-05-30
