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Product Info Summary
| SKU: | A01832 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-UBE2L3 Antibody
SKU/Catalog Number
A01832
Size
100ul
Form
Liquid
Description
Boster Bio Anti-UBE2L3 Antibody catalog # A01832. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE2L3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01832)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human UBE2L3(NP_001243284.1).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01832 is reactive to UBE2L3 in Human, Mouse, Rat
Calculated molecular weight
17.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01832 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IHC: 1:50-1:100
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using UBE2L3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 60s.
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Immunofluorescence analysis of HeLa cells using UBE2L3 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded rat lung using UBE2L3 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded rat kidney using UBE2L3 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Specific Publications For Anti-UBE2L3 Antibody (A01832)
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