Click image to see more details
Product Info Summary
| SKU: | A05874-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-UBE2T/Hspc150 Antibody
SKU/Catalog Number
A05874-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-UBE2T/Hspc150 Antibody catalog # A05874-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE2T/Hspc150 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05874-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human UBE2T. at AA rangle: 90-170
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05874-1 is reactive to UBE2T in Human, Mouse
Applications
A05874-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
22.521kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using UBE2T Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For UBE2T (Source: Uniprot.org, NCBI)
Gene Name
UBE2T
Full Name
Ubiquitin-conjugating enzyme E2 T
Weight
22.521kDa
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
Cell proliferation-inducing gene 50 protein; EC 6.3.2.19; HSPC150 protein similar to ubiquitin-conjugating enzyme; HSPC150; PIG50; UBE2T; Ubiquitin carrier protein T; ubiquitin conjugating enzyme; ubiquitin-conjugating enzyme E2 T; ubiquitin-conjugating enzyme E2T (putative); Ubiquitin-protein ligase T UBE2T FANCT, HSPC150, PIG50 ubiquitin conjugating enzyme E2 T ubiquitin-conjugating enzyme E2 T|E2 ubiquitin-conjugating enzyme T|HSPC150 protein similar to ubiquitin-conjugating enzyme|cell proliferation-inducing gene 50 protein|ubiquitin carrier protein T|ubiquitin conjugating enzyme E2T|ubiquitin-conjugating enzyme E2T (putative)|ubiquitin-protein ligase T
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBE2T, check out the UBE2T Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBE2T: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBE2T/Hspc150 Antibody (A05874-1)
Hello CJ!
No publications found for A05874-1
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-UBE2T/Hspc150 Antibody
Question
We are currently using anti-UBE2T/Hspc150 antibody A05874-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
M. Johnson
Verified customer
Asked: 2019-09-09
Answer
The anti-UBE2T/Hspc150 antibody (A05874-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-09
Question
Will A05874-1 anti-UBE2T/Hspc150 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
You can see on the product datasheet, A05874-1 anti-UBE2T/Hspc150 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-13
Question
I was wanting to use to test anti-UBE2T/Hspc150 antibody A05874-1 on human adrenal gland for research purposes, then I may be interested in using anti-UBE2T/Hspc150 antibody A05874-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Krishna
Verified customer
Asked: 2016-02-22
Answer
The products we sell, including anti-UBE2T/Hspc150 antibody A05874-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-02-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal gland using anti-UBE2T/Hspc150 antibody A05874-1. Let me know if you need anything else.
A. Dhar
Verified customer
Asked: 2013-11-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-18
