Anti-Ubiquitin Activating Enzyme E1 UBA1 Antibody

Product Name

Anti-Ubiquitin Activating Enzyme E1 UBA1 Antibody

View all Ubiquitin-activating Enzyme/UBE1 Antibodies

SKU/Catalog Number

A02810

Size

500ug

Form

Lyophilized

Description

Boster Bio Anti-Ubiquitin Activating Enzyme E1 UBA1 Antibody (Catalog # A02810). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Ubiquitin Activating Enzyme E1 UBA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02810)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Clone Number

EM-11

Isotype

IgG

Immunogen

Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.

Reactive Species

A02810 is reactive to UBA1 in Human

Applications

A02810 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

117.849kDa

Background of Ubiquitin-activating Enzyme/UBE1

Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 500 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: 2 mg/ml - 20 µg/ml
WB: 1:1,000 - 1:5,000

Validation Images & Assay Conditions

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UBA1 was detected in paraffin-embedded sections of human lung tissue using rabbit anti-Ubiquitin Activating Enzyme E1 polyclonal antibody (Catalog # A02810) at 10 μg/mL. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.

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Western blot analysis of UBA1 expression in A431 whole cell lysate. FBXL4 at 118KD was detected using rabbit anti-Ubiquitin Activating Enzyme E1 polyclonal antibody (Catalog # A02810) at 1:1000. Molecular weight markers are shown in lane 2 (700 nm channel). IRDye00 fluorescence image was captured using the Odyssey

Gene/Protein Information For UBA1 (Source: Uniprot.org, NCBI)

Alternative Names

A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; GXP1; MGC4781; POC20 centriolar protein homolog; POC20; Protein A1S9; SMAX2; UBA1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1A1S9; UBE1X; ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing); Ubiquitin-activating enzyme E1; Ubiquitinactivating Enzyme; Ubiquitin-activating Enzyme; ubiquitin-like modifier activating enzyme 1; ubiquitin-like modifier-activating enzyme 1 UBA1 A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2A, UBE1, UBE1X, VEXAS, UBA1 ubiquitin like modifier activating enzyme 1 ubiquitin-like modifier-activating enzyme 1|A1S9T and BN75 temperature sensitivity complementing|POC20 centriolar protein homolog|UBA1, ubiquitin-activating enzyme E1 homolog A|testicular secretory protein Li 63

For more info on UBA1, check out the UBA1 Infographic

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In this infographic, you will see the following information for UBA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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