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Product Info Summary
| SKU: | M02848-4 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, C. elegans, Chicken, Cow, Drosophila, Equine, Human, Monkey, Mouse, Pig, Rat, Horse |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ubiquitin Monoclonal Antibody
SKU/Catalog Number
M02848-4
Size
100ul
Description
Boster Bio Anti-Ubiquitin Monoclonal Antibody catalog # M02848-4. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, C. elegans, Chicken, Cow, Drosophila, Equine, Human, Monkey, Mouse, Pig, Rat, Horse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ubiquitin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02848-4)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Purified bovine erythrocyte ubiquitin coupled to KLH with glutaraldehyde
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02848-4 is reactive to UBA52 in Bovine, C. elegans, Chicken, Cow, Drosophila, Equine, Human, Monkey, Mouse, Pig, Rat, Horse
Applications
M02848-4 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
14.728kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000. ICC/IF and IHC: 1:2,000
Validation Images & Assay Conditions
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Western blot analysis of HEK293 cell lysates using mouse mAb to ubiquitin, M02848-4, dilution 1:1,000 in green. [1] protein standard (red), [2] cells maintained in normal medium, [3] cells treated with 10
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Formalin fixed paraffin embedded section of cerebral cortex of an Alzheimer patient processed with M02848-4 using HRP/DAB, giving a brown signal. Also stained with haemotoxylin in blue. A typical flame shaped tangle is seen in a pyramidal neuron in the center and is surrounded by some dystrophic neurites, also strongly ubiquitin positive. Both are commonly seen in cortical and hippocampal Alzheimer brain sections and are typical for this disease, but are rare or absent in healthy brain.
Protein Target Info & Infographic
Gene/Protein Information For UBA52 (Source: Uniprot.org, NCBI)
Gene Name
UBA52
Full Name
Ubiquitin-60S ribosomal protein L40
Weight
14.728kDa
Alternative Names
CEP52ubiquitin carboxyl extension protein 52; HUBCEP52; MGC126879; MGC126881; MGC57125; RPL40; UBCEP2; ubiquitin A-52 residue ribosomal protein fusion product 160S ribosomal protein L40; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52 UBA52 CEP52, HUBCEP52, L40, RPL40 ubiquitin A-52 residue ribosomal protein fusion product 1 ubiquitin-60S ribosomal protein L40|ubiquitin carboxyl extension protein 52|ubiquitin-52 amino acid fusion protein|ubiquitin-CEP52
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBA52, check out the UBA52 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBA52: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ubiquitin Monoclonal Antibody (M02848-4)
Hello CJ!
M02848-4 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mitochondrial E3 ubiquitin ligase 1 promotes brain injury by disturbing mitochondrial dynamics in a rat model of ischemic stroke
Biochemical Toxicity and Potential Detoxification Mechanisms in Earthworms Eisenia fetida Exposed to Sulfamethazine and Copper
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Ubiquitin Monoclonal Antibody
Question
We are currently using anti-Ubiquitin Monoclonal antibody M02848-4 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says bovine, c. elegans, chicken, drosophila, equine, mouse, pig. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The anti-Ubiquitin Monoclonal antibody (M02848-4) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-02
Question
Is a blocking peptide available for product anti-Ubiquitin Monoclonal antibody (M02848-4)?
K. Edwards
Verified customer
Asked: 2017-10-02
Answer
We do provide the blocking peptide for product anti-Ubiquitin Monoclonal antibody (M02848-4). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-02
Question
We want to test anti-Ubiquitin Monoclonal antibody M02848-4 on c. elegans fetal brain cortex for research purposes, then I may be interested in using anti-Ubiquitin Monoclonal antibody M02848-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Parker
Verified customer
Asked: 2015-10-21
Answer
The products we sell, including anti-Ubiquitin Monoclonal antibody M02848-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-10-21
