Anti-eRF1/ETF1 Antibody Picoband® (monoclonal, 3B6)

eRF1 antibody

Boster Bio Anti-eRF1/ETF1 Antibody Picoband® (monoclonal, 3B6) catalog # M04157. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: M04157
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-eRF1/ETF1 Antibody Picoband® (monoclonal, 3B6)

View all eRF1 Antibodies

SKU/Catalog Number

M04157

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-eRF1/ETF1 Antibody Picoband® (monoclonal, 3B6) catalog # M04157. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-eRF1/ETF1 Antibody Picoband® (monoclonal, 3B6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04157)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Monoclonal

Clone Number

3B6

Isotype

Mouse IgG2a

Immunogen

E.coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M04157 is reactive to ETF1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

49 kDa

Calculated molecular weight

49.031kDa

Background of eRF1

Eukaryotic translation termination factor 1 (eRF1), also known asTB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04157 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse, Rat

Positive Control

WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human Raji whole cell, human HEPG2 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell
IHC: human tonsil tissue, human ovarian cancer tissue, human breast cancer tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, rat kidney tissue
ICC/IF: MCF-7 cell
FCM: HEPA1-6 cell, CACO-2 cell, RH35 cell

Validation Images & Assay Conditions

Gene/Protein Information For ETF1 (Source: Uniprot.org, NCBI)

Gene Name

ETF1

Full Name

Eukaryotic peptide chain release factor subunit 1

Weight

49.031kDa

Superfamily

eukaryotic release factor 1 family

Alternative Names

AW546468 antibody; C80305 antibody; MGC18200 antibody; MGC4569 antibody; MGC54054 antibody; OTTHUMP00000147628 antibody; OTTHUMP00000229864 antibody; OTTHUMP00000229865 antibody; OTTHUMP00000229866 antibody; OTTHUMP00000229870 antibody; OTTHUMP00000229871 antibody; OTTHUMP00000229872 antibody; Placental ribonuclease inhibitor antibody; Placental RNase inhibitor antibody; PRI antibody; RAI antibody; RI antibody; Ribonuclease inhibitor antibody; Ribonuclease/angiogenin inhibitor 1 antibody; Ribonuclease/angiogenin inhibitor antibody; RINI_HUMAN antibody; RNase inhibitor antibody; RNH 1 antibody; RNH antibody; RNH1 antibody; Rnh1 ribonuclease/angiogenin inhibitor 1 antibody ETF1 D5S1995, ERF, ERF1, RF1, SUP45L1, TB3-1 eukaryotic translation termination factor 1 eukaryotic peptide chain release factor subunit 1|polypeptide chain release factor 1|protein Cl1|sup45 (yeast omnipotent suppressor 45) homolog-like 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ETF1, check out the ETF1 Infographic

ETF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ETF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M04157

100μg

$370
M04157-10ug

10μg sample (liquid)

$99
M04157-Biotin

100 μg Biotin conjugated

$570
M04157-Cy3

100 μg Cy3 conjugated

$570
M04157-Dylight488

100 μg Dylight488 conjugated

$570
M04157-Dylight550

100 μg Dylight550 conjugated

$570
M04157-Dylight594

100 μg Dylight594 conjugated

$570
M04157-FITC

100 μg FITC conjugated

$570
M04157-HRP

100 μg HRP conjugated

$570
M04157-APC

100 μg APC conjugated

$670
M04157-PE

100 μg PE conjugated

$670
M04157-iFluor647

100 μg iFluor647 conjugated

$670
M04157-carrier-free

Carrier Free

$370

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M04157
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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