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Product Info Summary
| SKU: | A05219-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ULK2 Antibody
SKU/Catalog Number
A05219-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ULK2 Antibody (Catalog # A05219-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
ULK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ULK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05219-1)
Host
Rabbit
Contents
ULK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ULK2 antibody was raised against a 17 amino acid peptide near the center of human ULK2 . The immunogen is located within amino acids 480 - 530 of ULK2.
Cross-reactivity
At least two isoforms of ULK2 are known to exist; this antibody will detect both isoforms. ULK2 antibody is predicted to not cross-react with ULK1.
Reactive Species
A05219-1 is reactive to ULK2 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
112.7 kDa
Background of ULK2
ULK2, also known as ATG1B, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK2 results in a severe defect in the autophagy pathway. ULK2 is highly conserved among eukaryotes and shows high homology with its related protein ULK1. Both ULK1 and ULK2 form a complex with ATG13 and FIP200 that mediates TOR signaling and is essential for autophagy. Like ULK1, ULK2 is also thought to be involved in early neuronal growth and differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05219-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ULK2 Antibody can be used for detection of ULK2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ULK2 in human brain tissue lysate with ULK2 antibody at 1 μg/mL.
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Immunohistochemistry of ULK2 in human brain tissue with ULK2 antibody at 2.5 μg/ml.
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Immunofluorescence of ULK2 in human brain tissue with ULK2 antibody at 20 μg/ml.
Specific Publications For Anti-ULK2 Antibody (A05219-1)
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