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Product Info Summary
| SKU: | A07706 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ULK3 Antibody
SKU/Catalog Number
A07706
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ULK3 Antibody (Catalog # A07706). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
ULK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ULK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07706)
Host
Rabbit
Contents
ULK3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ULK3 antibody was raised against a 19 amino acid peptide near the center of human ULK3. The immunogen is located within amino acids 230 - 280 of ULK3.
Cross-reactivity
ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.
Reactive Species
A07706 is reactive to ULK3 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
53.4 kDa
Background of ULK3
ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3). ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4). Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins; this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07706 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ULK3 antibody can be used for detection of ULK3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ULK3 in human brain tissue lysate with ULK3 antibody at (A) 0.5 and (B) 1 μg/ml.
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Immunohistochemistry of ULK3 in human brain tissue with ULK3 antibody at 5 μg/mL.
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Immunofluorescence of ULK3 in human brain tissue with ULK3 antibody at 20 μg/mL.
Specific Publications For Anti-ULK3 Antibody (A07706)
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