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Product Info Summary
| SKU: | A08552 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ULK4 Antibody
SKU/Catalog Number
A08552
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ULK4 Antibody (Catalog # A08552). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ULK4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ULK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08552)
Host
Rabbit
Contents
ULK4 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ULK4 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human ULK4. The immunogen is located within amino acids 1120 - 1170 of ULK4.
Cross-reactivity
ULK4 antibody is human and mouse reactive. Multiple isoforms of ULK4 are known to exist.
Reactive Species
A08552 is reactive to ULK4 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
142.4 kDa
Background of ULK4
ULK4 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK4, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK3, is highly conserved among eukaryotes (3). ULK4 has been implicated as a gene involved in the development of hydrocephalus (3). This neurologic disorder is being used in animal models to elucidate factors responsible for the excessive accumulation of cerebrospinal fluid in hydrocephalic humans (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08552 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ULK4 antibody can be used for detection of ULK4 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ULK4 in 3T3 cell lysate with ULK4 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of ULK4 in human brain tissue with ULK4 antibody at 5 μg/mL.
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Immunofluorescence of ULK4 in human brain tissue with ULK4 antibody at 20 μg/mL.
Specific Publications For Anti-ULK4 Antibody (A08552)
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