Anti-UPF3B/RENT3B Antibody Picoband™

Product Name

Anti-UPF3B/RENT3B Antibody Picoband™

View all UPF3B Antibodies

SKU/Catalog Number

PB9843

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-UPF3B/RENT3B Antibody Picoband™ catalog # PB9843. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-UPF3B/RENT3B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9843)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human UPF3B /RENT3B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9843 is reactive to UPF3B in Human, Mouse, Rat

Applications

PB9843 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

58 kDa

Calculated molecular weight

57.762kDa

Background of UPF3B

Regulator of nonsense transcripts 3B is a protein that in humans is encoded by the UPF3B gene. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 22RV1 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPF3B/RENT3B antigen affinity purified polyclonal antibody (Catalog # PB9843) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UPF3B/RENT3B at approximately 58 kDa. The expected band size for UPF3B/RENT3B is at 58 kDa.

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Figure 2. IHC analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843).
UPF3B/RENT3B was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UPF3B/RENT3B Antibody (PB9843) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843).
UPF3B/RENT3B was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UPF3B/RENT3B Antibody (PB9843) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 4. IHC analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843).
UPF3B/RENT3B was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UPF3B/RENT3B Antibody (PB9843) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For UPF3B (Source: Uniprot.org, NCBI)

Alternative Names

HUPF3B; Nonsense mRNA reducing factor 3B; regulator of nonsense transcripts 3B; RENT3BhUpf3p-X; UPF3 regulator of nonsense transcripts homolog B (yeast); UPF3XMRXS14; Up-frameshift suppressor 3 homolog B; Up-frameshift suppressor 3 homolog on chromosome X UPF3B HUPF3B, MRX62, MRX82, MRXS14, RENT3BP1, UPF3BP2, UPF3BP3, UPF3X, Upf3p-X, UPF3B UPF3B regulator of nonsense mediated mRNA decay regulator of nonsense transcripts 3B|UPF3 regulator of nonsense transcripts homolog B|UPF3B pseudogene 1|UPF3B pseudogene 2|UPF3B pseudogene 3|mental retardation, X-linked 62|nonfunctional UPF3B regulator of nonsense mediated mRNA decay|nonsense mRNA reducing factor 3B|up-frameshift suppressor 3 homolog B|up-frameshift suppressor 3 homolog on chromosome X

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In this infographic, you will see the following information for UPF3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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