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Product Info Summary
| SKU: | A05440 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-UROP11 Apba1 Antibody
SKU/Catalog Number
A05440
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-UROP11 Apba1 Antibody (Catalog # A05440). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
UROP11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-UROP11 Apba1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05440)
Host
Rabbit
Contents
UROP11 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
UROP11 antibody was raised against a 12 amino acid synthetic peptide near the center of the mouse UROP11. The immunogen is located within the first 50 amino acids of UROP11.
Reactive Species
A05440 is reactive to APBA1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
92.9 kDa
Background of APBA1
Upregulated obese product 11 (UROP11) was isolated from subtractive library for mouse hypothalamus. It is detected in numerous tissues and is upregulated in the hypothalamus and adipocytes in obese mice. UROP11 is also observed in human neural and lymphoid tissues; its expression appears to be regulated by the level of lymphoid activation. Urop11 expression is strongly upregulated both in vivo in mouse hypothalamus and in vitro in mouse neural cell lines after treatment with leptin, indicating Urop11 is a target of leptin activity and thus may play a role in obesity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05440 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
UROP11 antibody can be used for detection of UROP11 by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, UROP11 often migrates at a much higher apparent size in SDS-PAGE.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of UROP11 in mouse spleen tissue lysate with UROP11 antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-UROP11 Apba1 Antibody (A05440)
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