Anti-Uroplakin-1b UPK1B Antibody

Product Name

Anti-Uroplakin-1b UPK1B Antibody

View all UPK1B Antibodies

SKU/Catalog Number

A10230

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Uroplakin-1b UPK1B Antibody (Catalog # A10230). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

UPK1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Uroplakin-1b UPK1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10230)

Host

Rabbit

Contents

UPK1B Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

UPK1B antibody was raised against a 17 amino acid synthetic peptide near the center of human UPK1B. The immunogen is located within amino acids 110 - 160 of UPK1B.

Cross-reactivity

At least two isoforms of UPK1B are known to exist; this antibody will recognize both isoforms. UPK1B antibody is predicted to not cross-react with other tetraspanin protein family members.

Reactive Species

A10230 is reactive to UPK1B in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

29.6 kDa

Background of UPK1B

Uroplakin-1B (UPK1B) is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. UPK1B is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins and may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A10230 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

UPK1B antibody can be used for detection of UPK1B by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information UPK1B

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis