Product Info Summary
SKU: | A07816 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-USP13 Antibody
SKU/Catalog Number
A07816
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-USP13 Antibody (Catalog # A07816). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07816)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human USP13.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07816 is reactive to USP13 in Human, Mouse
Applications
A07816 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
97.327kDa
Background of USP13
Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
Timms K.M., Gene 217:101-106(1998).
Mural R.J., Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Dephoure N., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from mouse brain cells, using USP13 antibody A07816.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using USP13 antibody A07816.
Protein Target Info & Infographic
Gene/Protein Information For USP13 (Source: Uniprot.org, NCBI)
Gene Name
USP13
Full Name
Ubiquitin carboxyl-terminal hydrolase 13
Weight
97.327kDa
Superfamily
peptidase C19 family
Alternative Names
Deubiquitinating enzyme 13; EC 3.1.2.15; EC 3.4.19.12; Isopeptidase T-3; IsoT-3; ISOT3IsoT-3; ubiquitin carboxyl-terminal hydrolase 13; ubiquitin specific peptidase 13 (isopeptidase T-3); ubiquitin specific protease 13 (isopeptidase T-3); Ubiquitin Thioesterase 13; Ubiquitin thiolesterase 13; Ubiquitin-specific-processing protease 13; USP13 USP13 ISOT3, IsoT-3 ubiquitin specific peptidase 13 ubiquitin carboxyl-terminal hydrolase 13|deubiquitinating enzyme 13|ubiquitin specific peptidase 13 (isopeptidase T-3)|ubiquitin specific protease 13 (isopeptidase T-3)|ubiquitin thioesterase 13|ubiquitin thiolesterase 13|ubiquitin-specific-processing protease 13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP13, check out the USP13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP13 Antibody (A07816)
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No publications found for A07816
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