Click image to see more details
Product Info Summary
| SKU: | A13619 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-USP40 Antibody
SKU/Catalog Number
A13619
Size
100μl
Form
Liquid
Description
Boster Bio Anti-USP40 Antibody catalog # A13619. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP40 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13619)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human USP40. AA range:1011-1060
Reactive Species
A13619 is reactive to USP40 in Human, Mouse
Calculated molecular weight
140.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13619 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of 293 cells using USP40 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using USP40 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-USP40 Antibody (A13619)
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Customer Reviews
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7 Customer Q&As for Anti-USP40 Antibody
Question
Would anti-USP40 antibody A13619 work on primate WB with esophagus teratocarcinoma?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
Our lab technicians have not validated anti-USP40 antibody A13619 on primate. You can run a BLAST between primate and the immunogen sequence of anti-USP40 antibody A13619 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate esophagus teratocarcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-25
Question
Here is the WB image, lot number and protocol we used for esophagus teratocarcinoma using anti-USP40 antibody A13619. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-14
Question
Does A13619 anti-USP40 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Thomas
Verified customer
Asked: 2018-11-19
Answer
It shows on the product datasheet, A13619 anti-USP40 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus teratocarcinoma using anti-USP40 antibody A13619. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-27
Question
We are currently using anti-USP40 antibody A13619 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Z. Yang
Verified customer
Asked: 2015-12-01
Answer
The anti-USP40 antibody (A13619) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-01
Question
Is this A13619 anti-USP40 antibody reactive to the isotypes of USP40?
T. Jones
Verified customer
Asked: 2015-06-19
Answer
The immunogen of A13619 anti-USP40 antibody is Synthesized peptide derived from the Internal region of human USP40. at AA rangle: 980-1060. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-06-19
Question
I have a question about product A13619, anti-USP40 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Lewis
Verified customer
Asked: 2014-02-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13619 anti-USP40 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-02-05
