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Product Info Summary
| SKU: | M04720 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-VAC14 Antibody (N-Term)
SKU/Catalog Number
M04720
Size
50 µl
Description
Boster Bio Anti-VAC14 Antibody (N-Term) (Catalog # M04720). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-VAC14 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04720)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This VAC14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 125-159 amino acids from human VAC14.
Reactive Species
M04720 is reactive to VAC14 in Human
Calculated molecular weight
88.0 kDa
Background of VAC14
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04720 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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Anti-VAC14 Antibody (N-Term) at 1:2000 dilution + human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 88 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M04720 staining VAC14 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-VAC14 Antibody (N-Term) (M04720)
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