Anti-VCP Antibody

p97/VCP antibody

Boster Bio Anti-VCP Antibody catalog # PA2137. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Product Info Summary

SKU: PA2137
Size: 100 μg/vial
Reactive Species: Human, Monkey, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-VCP Antibody

View all p97/VCP Antibodies

SKU/Catalog Number

PA2137

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VCP Antibody catalog # PA2137. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VCP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2137)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human VCP, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2137 is reactive to VCP in Human, Monkey, Mouse, Rat

Applications

PA2137 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

89 kDa

Calculated molecular weight

89.322kDa

Background of p97/VCP

Valosin-containing protein also called CDC48 is an enzyme that in humans is encoded by the VCP gene. It is a member of the AAA+ (ATPase associated with various activities) protein family. The VCP gene maps to chromosome 9p13.3. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. It is involved in the formation of the transitional endoplasmic reticulum. This gene plays a role in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. It also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat
Immunocytochemistry , 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For VCP (Source: Uniprot.org, NCBI)

Gene Name

VCP

Full Name

Transitional endoplasmic reticulum ATPase

Weight

89.322kDa

Superfamily

AAA ATPase family

Alternative Names

15S Mg(2+)-ATPase p97 subunit; IBMPFD; IBMPFDMGC8560; MGC131997; p97; TER ATPase; TERA; transitional endoplasmic reticulum ATPase; valosin containing protein; Valosin-containing proteinMGC148092; VCP; yeast Cdc48p homolog VCP CDC48, FTDALS6, TERA, p97 valosin containing protein transitional endoplasmic reticulum ATPase|15S Mg(2+)-ATPase p97 subunit|TER ATPase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on VCP, check out the VCP Infographic

VCP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for VCP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-VCP Antibody

Question

I have a question about product PA2137, anti-VCP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2137 anti-VCP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-31

Question

We are interested in to test anti-VCP antibody PA2137 on mouse cerebellum for research purposes, then I may be interested in using anti-VCP antibody PA2137 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

The products we sell, including anti-VCP antibody PA2137, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-06

Question

Does anti-VCP antibody PA2137 work for IHC-P with cerebellum?

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

According to the expression profile of cerebellum, VCP is highly expressed in cerebellum. So, it is likely that anti-VCP antibody PA2137 will work for IHC-P with cerebellum.

Boster Scientific Support

Answered: 2019-11-12

Question

I see that the anti-VCP antibody PA2137 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-20

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-VCP antibody PA2137. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-06

Question

I was wanting to use your anti-VCP antibody for IHC-P for mouse cerebellum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cerebellum identification?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

As indicated on the product datasheet, PA2137 anti-VCP antibody has been tested for IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cerebellum in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-28

Question

Would PA2137 anti-VCP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Zhang

Verified customer

Asked: 2014-09-03

Answer

It shows on the product datasheet, PA2137 anti-VCP antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-09-03

Order DetailsPrice
PA2137

100μg

$370
PA2137-10ug

10μg sample (liquid)

$99
PA2137-Biotin

100 μg Biotin conjugated

$570
PA2137-Cy3

100 μg Cy3 conjugated

$570
PA2137-Dylight488

100 μg Dylight488 conjugated

$570
PA2137-Dylight550

100 μg Dylight550 conjugated

$570
PA2137-Dylight594

100 μg Dylight594 conjugated

$570
PA2137-FITC

100 μg FITC conjugated

$570
PA2137-HRP

100 μg HRP conjugated

$570
PA2137-APC

100 μg APC conjugated

$670
PA2137-PE

100 μg PE conjugated

$670
PA2137-carrier-free

Carrier Free

$370

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PA2137
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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