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Product Info Summary
| SKU: | A01168-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, IHC, WB |
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Product info
Product Name
Anti-VDAC1/2 Antibody
View all VDAC1/VDAC2 Antibodies
SKU/Catalog Number
A01168-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-VDAC1/2 Antibody catalog # A01168-2. Tested in WB, IHC, IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-VDAC1/2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01168-2)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human VDAC1/2 recombinant protein (Position: A2-E280).
Reactive Species
A01168-2 is reactive to VDAC1/VDAC2 in Human, Mouse, Rat
Background of VDAC1/VDAC2
The voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is thought to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. At low transmembrane voltage, VDAC is open for anions such as phosphate, chloride, and adenine nucleotides. At higher transmembrane voltage, VDAC functions as a selective channel for cations and uncharged molecules. These features make VDAC likely to play a role in mitochondrial energy metabolism. Huizing et al. studied by Northern and Western blot analyses the human tissue distribution of mitochondrial transmembrane metabolite carriers. They found that VDAC1 mRNA has a ubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas the VDAC2 isoform appears to be expressed only in the heart.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01168-2 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 1:500-2000 | |
| Immunohistochemistry | 1:50-400 | |
| Immunofluorescence | 1:50-400 | |
| Immunoprecipitation | 1:50 | |
| ELISA | 1:100-1000 |
Validation Images & Assay Conditions
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Western blot analysis of VDAC1/2 using anti-VDAC1/2 antibody (A01168-2).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDAC1/2 antigen affinity purified polyclonal antibody (A01168-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VDAC1/2 at approximately 31 kDa. The expected band size for VDAC1/2 is at 31 kDa.
Specific Publications For Anti-VDAC1/2 Antibody (A01168-2)
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