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VEGFA antibody (M00045-1) performs well in Flow Cytometry, IF, IHC, ICC and shows reactivity with Human, Mouse.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 18 Publications

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m00045 1 vegfa primary antibodies if testing 10

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IF analysis of VEGFA using anti-VEGFA antibody (M00045-1).
VEGFA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-VEGFA Antibody (M00045-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-VEGF VEGFA Rabbit Monoclonal Antibody

VEGFA antibody

18 Publications

Reactive Species	Host	Application
Human, Mouse	Rabbit	Flow Cytometry, IF, IHC, ICC

Tested Applications

VEGFA antibody was positively detected by IHC in:

human kindey tissue, human breast cancer tissue, human colon cancer tissue, human endometrial cancer tissue, mouse kidney tissue, mouse pancreas tissue, human ovarian cancer tissue, human prostatic cancer tissue, rat thyroid tissue, human thyroid cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

VEGFA antibody was positively detected by IF in:

human breast cancer tissue, human endometrial cancer tissue, human ovarian cancer tissue, human pancreatic cancer tissue, human prostatic cancer tissue, human thyroid cancer tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Tested

Recommend

Mouse

Tested

Recommend

Rat

Tested

Recommend

Product Information

Product Name

Anti-VEGF VEGFA Rabbit Monoclonal Antibody
View all VEGFA Antibodies

Form

Liquid

Uniprot

P15692

Gene ID

7422

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

ADB-22

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human VEGF

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

28 kDa

Calculated Molecular Weight

27.0 kDa

Cite This Product

Anti-VEGF VEGFA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00045-1)

Protocols

Immunohistochemistry (IHC) protocol for VEGFA antibody

Review Protocol

Immunofluorescence (IF) protocol for VEGFA antibody

Review Protocol

Publications

Histologic and immunohistochemical changes in amputated and scarring lizard limb of Scincella tsinlingensis

Published: 2026-03-12

Journal: ZOOLOGY

Application: IHC

Impact Factor: 1.60

Species: Scincella tsinlingensis

PMID: 41850143

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Free Secondary Antibody

Anti-VEGF VEGFA Rabbit Monoclonal Antibody

VEGFA antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Histologic and immunohistochemical changes in amputated and scarring lizard limb of Scincella tsinlingensis

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Multitargeting Strategy Using Tetrathiomolybdate and Lenvatinib: Maximizing Antiangiogenesis Activity in a Preclinical Liver Cancer Model.

NIR-activated multi-hit therapeutic Ag2S quantum dot-based hydrogel for healing of bacteria-infected wounds

Endocrine, Stemness, Proliferative, and Proteolytic Properties of Alarm Cells in Ruby-Red-Fin Shark (Rainbow Shark), Epalzeorhynchos frenatum (Teleostei: Cyprinidae)

Development of Poloxamer Hydrogels Containing Antibacterial Guanidine-Based Polymers for Healing of Full-Thickness Skin Wound

The overexpression of uPA promotes the proliferation and fibrinolytic activity of human umbilical vein endothelial cells

Ginsenoside Rb3 exerts protective properties against cigarette smoke extract-induced cell injury by inhibiting the p38 MAPK/NF-κB and TGF-β1/VEGF pathways in fibroblasts and epithelial cells

Systemic leptin administration alters callus VEGF levels and enhances bone fracture healing in wildtype and ob/ob mice

Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head

Effect of Melilotus suaveolens extract on pulmonary microvascular permeability by downregulating vascular endothelial growth factor expression in rats with sepsis

Interleukin-18 suppresses angiogenesis and lymphangiogenesis in implanted Lewis lung cancer

Review

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