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Product Info Summary
| SKU: | A07775-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, IHC, WB |
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Product info
Product Name
Anti-VGAT/SLC32A1 Antibody
SKU/Catalog Number
A07775-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-VGAT/SLC32A1 Antibody catalog # A07775-1. Tested in WB, IHC, IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-VGAT/SLC32A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07775-1)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human VGAT/SLC32A1 recombinant protein (Position: T3-A121).
Reactive Species
A07775-1 is reactive to SLC32A1 in Human, Mouse, Rat
Observed Molecular Weight
57 kDa
Calculated molecular weight
57.4 kDa
Background of SLC32A1
SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07775-1 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunofluorescence, 1:50-400
ImmunoPrecipitation, 1:250-300
ELISA, 1:100-1000
Positive Control
WB: rat brain tissue lysates, mouse brain tissue lysates, mouse eye tissue lysates
Validation Images & Assay Conditions
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Western blot analysis of VGAT/SLC32A1 using anti-VGAT/SLC32A1 antibody (A07775-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: mouse eye tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VGAT/SLC32A1 antigen affinity purified polyclonal antibody (A07775-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VGAT/SLC32A1 at approximately 57 kDa. The expected band size for VGAT/SLC32A1 is at 57 kDa.
Specific Publications For Anti-VGAT/SLC32A1 Antibody (A07775-1)
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