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VIM antibody (PB9359) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 88 Publications

pb9359 vimentin primary antibodies if testing 11

IF analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-Vimentin Antibody Picoband®

Name: Anti-Vimentin Antibody Picoband®
Brand: Boster Bio
SKU: PB9359
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

VIM antibody

88 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IF, IHC, WB

Tested Applications

VIM antibody was positively detected by WB in:

human placenta tissue, human U-87MG whole cell, human U20S whole cell, human PC-3 whole cell, human Hela whole cell, human THP-1 whole cell, human A549 whole cell, human MCF-7 whole cell, rat ovary tissue, rat lung tissue, mouse lung tissue, mouse NIH/3T3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

VIM antibody was positively detected by IHC in:

mouse cardiac muscle tissue, rat cardiac muscle tissue, human mammary cancer tissue, human placenta tissue, human lung adenocarcinoma tissue, human colorectal adenocarcinoma tissue, human invasive urothelial carcinoma tissue, human liver cancer tissue, human tonsil tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

VIM antibody was positively detected by IF in:

mouse brain tissue, rat brain tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Mouse

Tested

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-Vimentin Antibody Picoband®
View all VIM Antibodies

Form

Lyophilized

Uniprot

P08670

Gene ID

7431

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin, identical to the related mouse and rat sequences.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

54 kDa

Calculated Molecular Weight

53.7 kDa

Background of VIM

VIM (vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Cite This Product

Anti-Vimentin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9359)

Protocols

Western Blot protocol for VIM antibody

Review Protocol

Immunohistochemistry (IHC) protocol for VIM antibody

Review Protocol

Immunofluorescence (IF) protocol for VIM antibody

Review Protocol

Publications

CBLC-mediated ubiquitination stabilizes METTL1, enhances N7-methylguanosine modification of ESRRA, and promotes the progression of endometrial cancer

Published: 2026-05-30

Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Application: WB

Impact Factor: 8.70

Species: Human

PMID: 42217705

SKU	PB9359
Application	Western Blot
Sample	human HTR8 cell
Sample Processing Description	The samples were lysed in RIPA buffer, mixed with β-mercaptoethanol, and denatured at 100°C for 10 minutes before loading onto an SDS-PAGE gel.
Other Reagents	5% Non-fat milk
Primary Antibody	Vimentin Antibody Picoband®
Primary Incubation	1:2500, overnight at 4 ℃
Secondary Antibody	HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation	1 hour in room temperature
Detection	Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary	This antibody is highly specific and efficient, suitable for detecting Vimentin protein in HTR8 cells by Western blot, with only minor nonspecific bands observed.

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Anti-Vimentin Antibody Picoband®

VIM antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

CBLC-mediated ubiquitination stabilizes METTL1, enhances N7-methylguanosine modification of ESRRA, and promotes the progression of endometrial cancer

MYC promotes myocardial fibrosis via METTL1-mediated m7G modification of HILPDA

Mechanical-biochemical signaling accelerates scleral remodeling in myopia via the LaminA/C-Emerin-MKL1 axis

GABPB1 activates transcription of ITPR3 and EMT in OSCC progression

Study on the mechanism of PSENEN in modulating cisplatin resistance in NSCLC through stromal cell interactions

Apoptosis-promoting autophagy mediates anti-migratory effects of unsymmetrical bisacridines in pancreatic cancer cells

Ustiloxins induce kidney injury via the TLR2/MAPK/NF-κB pathway

Development of an Effective Drug Carrier for Targeted Therapy in Lung Adenocarcinoma Using ROS-responsive Micelles

Yak FGF12 promotes the proliferation of fibroblasts and longissimus lumborum development

N-terminomics and proteomics analysis of Calpain-2 reveal key proteolytic processing of metabolic and cell adhesion proteins

Piezo1 Regulates Odontogenesis via a FAM83G-Mediated Mechanism in Dental Papilla Cells In Vitro and In Vivo

Treatment of rheumatoid arthritis using dual-targeted and dual-response intelligent micelles: a “three birds with one stone” strategy

Ectopic endometrial stromal cell-derived extracellular vesicles encapsulating microRNA-25-3p induce endometrial collagen I deposition impairing decidualization in endometriosis

Synergistic effects of bloom helicase (BLM) inhibitor AO/854 with cisplatin in prostate cancer

LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts

Construction of Lentiviral Vectors Carrying Six Pluripotency Genes in Yak to Obtain Yak iPSC Cells

Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma

METTL3-dependent m6A modification facilitates decreased endometrial decidualization via attenuation MET in endometriosis

Inhibition of Notch3/Hey1 ameliorates peribiliary hypoxia by preventing hypertrophic hepatic arteriopathy in biliary atresia progression

PCSK5 downregulation promotes the inhibitory effect of andrographolide on glioblastoma through regulating STAT3

CircFOXO3 mediates hypoxia-induced autophagy of endometrial stromal cells in endometriosis

Juan-tong-yin potentially impacts endometriosis pathophysiology by enhancing autophagy of endometrial stromal cells via unfolded protein reaction-triggered endoplasmic reticulum stress

Micafungin exerts antitumor effect on breast cancer and osteosarcoma through preventing EMT in tumor cells in an USP7/AKT/GSK-3β pathway-dependent manner

Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration

Downregulation of MAL2 inhibits breast cancer progression through regulating β-catenin/c-Myc axis

CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling

Effects of norepinephrine‑induced activation of rat vascular adventitial fibroblasts on proliferation and migration of BMSCs involved in vascular remodeling

Hepatocyte-targeted delivery using oleanolic acid-loaded liposomes for enhanced hepatocellular carcinoma therapy

Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human gingival fibroblasts and human gingival epithelial cells

Litchi procyanidins inhibit colon cancer proliferation and metastasis by triggering gut-lung axis immunotherapy

Discovery of a Novel Bloom’s Syndrome Protein (BLM) Inhibitor Suppressing Growth and Metastasis of Prostate Cancer

Gentiopicroside Ameliorates Diabetic Renal Tubulointerstitial Fibrosis via Inhibiting the AT1R/CK2/NF-κB Pathway

Effects of Huangqi Liuyi Decoction in the Treatment of Diabetic Nephropathy and Tissue Distribution Difference of its Six Active Constituents Between Normal and Diabetic Nephropathy Mouse Models

Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter

The Therapeutic Effects of Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells on Scleroderma

Effect of Porphyromonas gingivalis lipopolysaccharide on calcification of human umbilical artery smooth muscle cells co-cultured with human periodontal ligament cells

MicroRNA‑181a promotes epithelial‑mesenchymal transition in esophageal squamous cell carcinoma via the TGF‑β/Smad pathway

Elevated Circular RNA PVT1 Promotes Eutopic Endometrial Cell Proliferation and Invasion of Adenomyosis via miR-145/Talin1 Axis

SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions

LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-145 inhibition in gastric cancer

Baicalein inhibits inflammatory response and promotes osteogenic activity in periodontal ligament cells challenged with lipopolysaccharides

STAT1-induced regulation of lncRNA ZFPM2-AS1 predicts poor prognosis and contributes to hepatocellular carcinoma progression via the miR-653/GOLM1 axis

RETRACTED: BMSCs-derived exosomal microRNA-let-7a plays a protective role in diabetic nephropathy via inhibition of USP22 expression

Mechanism of transmembrane and coiled‑coil domain 1 in the regulation of proliferation and migration of A549 cells

Infection of Placental Extravillous Cytotrophoblasts with Human Cytomegalovirus Causes a Treg/Th17 Imbalance at the Maternal–Fetal Interface

Tumor-associated macrophages promote prostate cancer progression via exosome-mediated miR-95 transfer

RETRACTED ARTICLE: STAT1-induced upregulation of lncRNA LINC01123 predicts poor prognosis and promotes the progression of endometrial cancer through miR-516b/KIF4A

Endosulfan triggers epithelial-mesenchymal transition via PTP4A3-mediated TGF-β signaling pathway in prostate cancer cells

Comprehensive circular RNA profiling reveals the regulatory role of circRNA_0007694 in papillary thyroid carcinoma

Development and Radiation Response Assessment in A Novel Syngeneic Mouse Model of Tongue Cancer: 2D Culture, 3D Organoids and Orthotopic Allografts

LRRC4 Suppresses E-Cadherin-Dependent Collective Cell Invasion and Metastasis in Epithelial Ovarian Cancer

Decreased abundance of GDNF mRNA transcript in the immature Sertoli cells of cattle in response to protein kinase inhibitor staurosporine

Biological and Proteomic Characteristics of an Immortalized Human Pancreatic Stellate Cell Line

Antitumor activity of a novel dual functional podophyllotoxin derivative involved PI3K/AKT/mTOR pathway

TAZ contributes to osteogenic differentiation of periodontal ligament cells under tensile stress

Comparison of Rapidly Proliferating, Multipotent Aortic Valve-Derived Stromal Cells and Valve Interstitial Cells in the Human Aortic Valve

Sohlh2 alleviates malignancy of EOC cells under hypoxia via inhibiting the HIF1α/CA9 signaling pathway

p53-Mediated PI3K/AKT/mTOR Pathway Played a Role in PtoxDpt-Induced EMT Inhibition in Liver Cancer Cell Lines

MicroRNA-139 inhibits hepatocellular carcinoma cell growth through down-regulating karyopherin alpha 2

Hsa_circ_0005075 predicts a poor prognosis and acts as an oncogene in colorectal cancer via activating Wnt/β-catenin pathway

MDM2 promotes epithelial–mesenchymal transition through activation of Smad2/3 signaling pathway in lung adenocarcinoma

miR-130a-3p regulated TGF-β1-induced epithelial-mesenchymal transition depends on SMAD4 in EC-1 cells

Hypoxia-induced LncRNA PCGEM1 promotes invasion and metastasis of gastric cancer through regulating SNAI1

Up-regulation of E-cadherin by saRNA inhibits the migration and invasion of renal carcinoma cells

Interleukin-27 Inhibits Trophoblast Cell Invasion and Migration by Affecting the Epithelial–Mesenchymal Transition in Preeclampsia

RSPO2 enhances cell invasion and migration via the WNT/β-catenin pathway in human gastric cancer

MicroRNA-3619-5p suppresses bladder carcinoma progression by directly targeting β-catenin and CDK2 and activating p21