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Product Info Summary
| SKU: | A04345 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-VIP Receptor 1/VIPR1 Antibody Picoband®
SKU/Catalog Number
A04345
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VIP Receptor 1/VIPR1 Antibody Picoband® catalog # A04345. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VIP Receptor 1/VIPR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04345)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human VIP Receptor 1, which shares 84% amino acid (aa) sequence identity with both mouse and rat VIP Receptor 1.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04345 is reactive to VIPR1 in Human, Mouse, Rat
Observed Molecular Weight
52 kDa
Calculated molecular weight
51.5 kDa
Background of VIPR1
VIPR1 (Vasoactive intestinal polypeptide receptor 1),also known as VIPR,HVR1, is a protein that in humans is encoded by the VIPR1 gene. Distinct subsets of neural, respiratory, gastrointestinal, and immune cells bear specific high-affinity G protein-coupled receptors for VIP, such as VIPR1. The VIPR1 gene is mapped on 3p22.1. The VIPR1 gene was found to span approximately 22 kb and to be comprised of 13 exons (ranging from 42 to 1,400 bp) and 12 introns (ranging from 0.3 to 6.1 kb). One encodes a VIP receptor consisting of 460 amino acids and having 7 putative transmembrane domains, as do other G protein-coupled receptors. Patients with idiopathic achalasia show a significant difference in the distribution of SNPs affecting VIPR1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04345 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Positive Control
WB: human A431 cell, human PANC-1 cell
Validation Images & Assay Conditions
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Western blot analysis of VIP Receptor 1 using anti-VIP Receptor 1 antibody (A04345).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A431 cell lysates,
Lane 2: human PANC-1 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VIP Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A04345) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VIP Receptor 1 at approximately 52KD. The expected band size for VIP Receptor 1 is at 52KD.
Specific Publications For Anti-VIP Receptor 1/VIPR1 Antibody Picoband® (A04345)
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