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- Table of Contents
VSIR antibody (A32320) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of VISTA in human tonsil tissue with B7-H5 antibody at 20 μg/ml.
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Anti-VISTA VSIR Antibody
VSIR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IF, IHC, ICC, WB |
Recommend Dilution
Application
Dilution
Species
VISTA antibody can be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL. For Immunocytochemistry start at 1 μg/mL.
Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-VISTA VSIR Antibody
View all VSIR Antibodies
View all VSIR Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
VISTA antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
VISTA Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
VISTA antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human VISTA. The immunogen is located within the last 60 amino acids of VISTA.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
33.9 kDa
Background of VSIR
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).
Cite This Product
Anti-VISTA VSIR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32320)
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Product Promise
We promise Anti-VISTA VSIR Antibody performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A32320
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026