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Product Info Summary
| SKU: | A02232-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-VMAT2/SLC18A2 Antibody Picoband®
SKU/Catalog Number
A02232-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VMAT2/SLC18A2 Antibody Picoband® catalog # A02232-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-VMAT2/SLC18A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02232-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human VMAT2/SLC18A2 recombinant protein (Position: M1-D514).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02232-1 is reactive to SLC18A2 in Human, Rat
Observed Molecular Weight
75 kDa
Calculated molecular weight
55.7 kDa
Background of SLC18A2
This gene encodes an transmembrane protein that functions as an ATP-dependent transporter of monoamines, such as dopamine, norepinephrine, serotonin, and histamine. This protein transports amine neurotransmitters into synaptic vesicles. Polymorphisms in this gene may be associated with schizophrenia, bipolar disorder, and other neurological/psychiatric ailments.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02232-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of VMAT2/SLC18A2 using anti-VMAT2/SLC18A2 antibody (A02232-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VMAT2/SLC18A2 antigen affinity purified polyclonal antibody (Catalog # A02232-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VMAT2/SLC18A2 at approximately 75 kDa. The expected band size for VMAT2/SLC18A2 is at 56 kDa.
Specific Publications For Anti-VMAT2/SLC18A2 Antibody Picoband® (A02232-1)
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