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Product Info Summary
| SKU: | A12325 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-VPS26B Antibody
SKU/Catalog Number
A12325
Size
100μl
Form
Liquid
Description
Boster Bio Anti-VPS26B Antibody catalog # A12325. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VPS26B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12325)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human VPS26B. AA range:231-280
Reactive Species
A12325 is reactive to VPS26B in Human, Mouse
Calculated molecular weight
39.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12325 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of LOVO cells using VPS26B Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysates from LOVO cells, using VPS26B Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-VPS26B Antibody (A12325)
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Customer Q&As
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7 Customer Q&As for Anti-VPS26B Antibody
Question
Would anti-VPS26B antibody A12325 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
According to the expression profile of cervix carcinoma, VPS26B is highly expressed in cervix carcinoma. So, it is likely that anti-VPS26B antibody A12325 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-08-13
Question
We want to test anti-VPS26B antibody A12325 on human cervix carcinoma for research purposes, then I may be interested in using anti-VPS26B antibody A12325 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The products we sell, including anti-VPS26B antibody A12325, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-20
Question
Does anti-VPS26B antibody A12325 work on pig WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
Our lab technicians have not tested anti-VPS26B antibody A12325 on pig. You can run a BLAST between pig and the immunogen sequence of anti-VPS26B antibody A12325 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-06
Question
Is there a BSA free version of anti-VPS26B antibody A12325 available?
H. Dhar
Verified customer
Asked: 2018-08-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VPS26B antibody A12325 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-23
Question
Would A12325 anti-VPS26B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Baker
Verified customer
Asked: 2018-07-19
Answer
You can see on the product datasheet, A12325 anti-VPS26B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-19
Question
Is a blocking peptide available for product anti-VPS26B antibody (A12325)?
E. Roberts
Verified customer
Asked: 2018-02-22
Answer
We do provide the blocking peptide for product anti-VPS26B antibody (A12325). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-02-22
Question
We are currently using anti-VPS26B antibody A12325 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
The anti-VPS26B antibody (A12325) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-11
