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Product Info Summary
| SKU: | A01644-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-VPS35 Antibody
SKU/Catalog Number
A01644-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-VPS35 Antibody catalog # A01644-1. Tested in WB,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VPS35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01644-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human TPH2 around the phosphorylation site of S19.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01644-1 is reactive to VPS35 in Human, Mouse
Applications
A01644-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
91.707kDa
Background of VPS35
TYK2 encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with hyperimmunoglobulin E syndrome (HIES) - a primary immunodeficiency characterized by elevated serum immunoglobulin E.
Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Gauzzi MC, et al. (1996) J Biol Chem. 271(34): 20494-20500.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
IHC: 1:100-1:300
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of VPS35 using Anti-VPS35 Antibody (A01644-1).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-VPS35 Antibody A01644-1
Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.Positive control:
Lane 1: PC-3M
Lane 2: U937
Lane 3: SH-SY-5Y
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For VPS35 (Source: Uniprot.org, NCBI)
Gene Name
VPS35
Full Name
Vacuolar protein sorting-associated protein 35
Weight
91.707kDa
Superfamily
VPS35 family
Alternative Names
DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; Maternal-embryonic 3; MEM3DKFZp434E1211; vacuolar protein sorting 35 (yeast homolog); vacuolar protein sorting 35 (yeast); vacuolar protein sorting 35 homolog (S. cerevisiae); vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35 VPS35 MEM3, PARK17 VPS35 retromer complex component vacuolar protein sorting-associated protein 35|hVPS35|maternal-embryonic 3|vacuolar protein sorting 35 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VPS35, check out the VPS35 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VPS35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VPS35 Antibody (A01644-1)
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No publications found for A01644-1
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