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Product Info Summary
| SKU: | M01644 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-VPS35 Monoclonal Antibody
SKU/Catalog Number
M01644
BM5543 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-VPS35 Monoclonal Antibody catalog # M01644. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VPS35 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01644)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AECH-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human VPS35
Reactive Species
M01644 is reactive to VPS35 in Human, Mouse, Rat
Observed Molecular Weight
92 kDa
Calculated molecular weight
91.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01644 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: HeLa cell, Mouse kidney
Validation Images & Assay Conditions
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Western blot analysis of VPS35 expression in (1) HeLa cell lysate; (2) Mouse kidney lysate.
Specific Publications For Anti-VPS35 Monoclonal Antibody (M01644)
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3 Customer Q&As for Anti-VPS35 Monoclonal Antibody
Question
We are currently using anti-VPS35 Monoclonal antibody M01644 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
G. Zhang
Verified customer
Asked: 2019-12-16
Answer
The anti-VPS35 Monoclonal antibody (M01644) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-16
Question
Does anti-VPS35 Monoclonal antibody M01644 work for IF with pheochromocytoma?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
According to the expression profile of pheochromocytoma, VPS35 is highly expressed in pheochromocytoma. So, it is likely that anti-VPS35 Monoclonal antibody M01644 will work for IF with pheochromocytoma.
Boster Scientific Support
Answered: 2019-07-22
Question
Will anti-VPS35 Monoclonal antibody M01644 work on dog WB with colon?
C. Dhar
Verified customer
Asked: 2013-01-08
Answer
Our lab technicians have not tested anti-VPS35 Monoclonal antibody M01644 on dog. You can run a BLAST between dog and the immunogen sequence of anti-VPS35 Monoclonal antibody M01644 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-08
