Anti-VPS35 Monoclonal Antibody

VPS35 antibody

Boster Bio Anti-VPS35 Monoclonal Antibody catalog # M01644. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01644
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-VPS35 Monoclonal Antibody

View all VPS35 Antibodies

SKU/Catalog Number

M01644

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-VPS35 Monoclonal Antibody catalog # M01644. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VPS35 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01644)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AECH-22

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human VPS35

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01644 is reactive to VPS35 in Human, Mouse, Rat

Applications

M01644 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

91.707kDa

Background of VPS35

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For VPS35 (Source: Uniprot.org, NCBI)

Gene Name

VPS35

Full Name

Vacuolar protein sorting-associated protein 35

Weight

91.707kDa

Superfamily

VPS35 family

Alternative Names

DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; Maternal-embryonic 3; MEM3DKFZp434E1211; vacuolar protein sorting 35 (yeast homolog); vacuolar protein sorting 35 (yeast); vacuolar protein sorting 35 homolog (S. cerevisiae); vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35 VPS35 MEM3, PARK17 VPS35 retromer complex component vacuolar protein sorting-associated protein 35|hVPS35|maternal-embryonic 3|vacuolar protein sorting 35 homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on VPS35, check out the VPS35 Infographic

VPS35 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for VPS35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-VPS35 Monoclonal Antibody

Question

We are currently using anti-VPS35 Monoclonal antibody M01644 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

G. Zhang

Verified customer

Asked: 2019-12-16

Answer

The anti-VPS35 Monoclonal antibody (M01644) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-16

Question

Does anti-VPS35 Monoclonal antibody M01644 work for IF with pheochromocytoma?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

According to the expression profile of pheochromocytoma, VPS35 is highly expressed in pheochromocytoma. So, it is likely that anti-VPS35 Monoclonal antibody M01644 will work for IF with pheochromocytoma.

Boster Scientific Support

Answered: 2019-07-22

Question

Will anti-VPS35 Monoclonal antibody M01644 work on dog WB with colon?

C. Dhar

Verified customer

Asked: 2013-01-08

Answer

Our lab technicians have not tested anti-VPS35 Monoclonal antibody M01644 on dog. You can run a BLAST between dog and the immunogen sequence of anti-VPS35 Monoclonal antibody M01644 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog colon in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-01-08

Order DetailsPrice
M01644

100μL

$370
M01644-30ul

30μL sample

$99
M01644-carrier-free

Carrier Free

$370

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M01644
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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