Product Info Summary
SKU: | M01644 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-VPS35 Monoclonal Antibody
SKU/Catalog Number
M01644
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-VPS35 Monoclonal Antibody catalog # M01644. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VPS35 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01644)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AECH-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human VPS35
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01644 is reactive to VPS35 in Human, Mouse, Rat
Applications
M01644 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
80 kDa
Calculated molecular weight
91.707kDa
Background of VPS35
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of VPS35 expression in (1) HeLa cell lysate; (2) Mouse kidney lysate.
Protein Target Info & Infographic
Gene/Protein Information For VPS35 (Source: Uniprot.org, NCBI)
Gene Name
VPS35
Full Name
Vacuolar protein sorting-associated protein 35
Weight
91.707kDa
Superfamily
VPS35 family
Alternative Names
DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; Maternal-embryonic 3; MEM3DKFZp434E1211; vacuolar protein sorting 35 (yeast homolog); vacuolar protein sorting 35 (yeast); vacuolar protein sorting 35 homolog (S. cerevisiae); vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35 VPS35 MEM3, PARK17 VPS35 retromer complex component vacuolar protein sorting-associated protein 35|hVPS35|maternal-embryonic 3|vacuolar protein sorting 35 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VPS35, check out the VPS35 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VPS35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VPS35 Monoclonal Antibody (M01644)
Hello CJ!
No publications found for M01644
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-VPS35 Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-VPS35 Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-VPS35 Monoclonal Antibody
Question
We are currently using anti-VPS35 Monoclonal antibody M01644 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
G. Zhang
Verified customer
Asked: 2019-12-16
Answer
The anti-VPS35 Monoclonal antibody (M01644) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-16
Question
Does anti-VPS35 Monoclonal antibody M01644 work for IF with pheochromocytoma?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
According to the expression profile of pheochromocytoma, VPS35 is highly expressed in pheochromocytoma. So, it is likely that anti-VPS35 Monoclonal antibody M01644 will work for IF with pheochromocytoma.
Boster Scientific Support
Answered: 2019-07-22
Question
Will anti-VPS35 Monoclonal antibody M01644 work on dog WB with colon?
C. Dhar
Verified customer
Asked: 2013-01-08
Answer
Our lab technicians have not tested anti-VPS35 Monoclonal antibody M01644 on dog. You can run a BLAST between dog and the immunogen sequence of anti-VPS35 Monoclonal antibody M01644 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-08