Anti-MEK5 Rabbit Monoclonal Antibody

MEK5 antibody

Boster Bio Anti-MEK5 Rabbit Monoclonal Antibody catalog # M03980. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Product Info Summary

SKU: M03980
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, ICC, WB

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Product Name

Anti-MEK5 Rabbit Monoclonal Antibody

View all MEK5 Antibodies

SKU/Catalog Number

M03980

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-MEK5 Rabbit Monoclonal Antibody catalog # M03980. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MEK5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03980)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

HCE-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human MEK5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M03980 is reactive to MAP2K5 in Human

Applications

M03980 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

50.112kDa

Background of MEK5

Plays a major role in tight junction-specific obliteration of the intercellular space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For MAP2K5 (Source: Uniprot.org, NCBI)

Gene Name

MAP2K5

Full Name

Dual specificity mitogen-activated protein kinase kinase 5

Weight

50.112kDa

Superfamily

protein kinase superfamily

Alternative Names

HsT17454; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAP2K5; MAPK/ERK kinase 5; MAPKK 5; MAPKK5; MAPKK5EC 2.7.12.2; MEK5; MEK5MEK 5; mitogen-activated protein kinase kinase 5; MKK5; PRKMK5; PRKMK5dual specificity mitogen-activated protein kinase kinase 5 MAP2K5 HsT17454, MAPKK5, MEK5, PRKMK5 mitogen-activated protein kinase kinase 5 dual specificity mitogen-activated protein kinase kinase 5|MAP kinase kinase 5|MAP kinase kinase MEK5b|MAPK/ERK kinase 5|MAPKK 5|MEK 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAP2K5, check out the MAP2K5 Infographic

MAP2K5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAP2K5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-MEK5 Rabbit Monoclonal Antibody

Question

Is this M03980 anti-MEK5 Rabbit Monoclonal antibody reactive to the isotypes of MAP2K5?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

The immunogen of M03980 anti-MEK5 Rabbit Monoclonal antibody is A synthesized peptide derived from human MEK5 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-20

Question

My question regarding product M03980, anti-MEK5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03980 anti-MEK5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-11

Question

Do you have a BSA free version of anti-MEK5 Rabbit Monoclonal antibody M03980 available?

Verified Customer

Verified customer

Asked: 2019-05-30

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MEK5 Rabbit Monoclonal antibody M03980 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-30

Question

I was wanting to use your anti-MEK5 Rabbit Monoclonal antibody for WB for human cerebellum on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cerebellum identification?

Verified Customer

Verified customer

Asked: 2018-09-21

Answer

As indicated on the product datasheet, M03980 anti-MEK5 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cerebellum in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-21

Question

I am interested in to test anti-MEK5 Rabbit Monoclonal antibody M03980 on human cerebellum for research purposes, then I may be interested in using anti-MEK5 Rabbit Monoclonal antibody M03980 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-04-05

Answer

The products we sell, including anti-MEK5 Rabbit Monoclonal antibody M03980, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-04-05

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-MEK5 Rabbit Monoclonal antibody M03980. Let me know if you need anything else.

G. Carter

Verified customer

Asked: 2016-07-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-07-06

Question

I see that the anti-MEK5 Rabbit Monoclonal antibody M03980 works with WB, what is the protocol used to produce the result images on the product page?

R. Bhatt

Verified customer

Asked: 2015-08-04

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-08-04

Order DetailsPrice
M03980

100μL

$370
M03980-30ul

30μL sample

$99
M03980-carrier-free

Carrier Free

$370

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M03980
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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