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Product Info Summary
| SKU: | M03980 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-MEK5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03980
BM4705 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MEK5 Rabbit Monoclonal Antibody catalog # M03980. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03980)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HCE-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MEK5
Reactive Species
M03980 is reactive to MAP2K5 in Human
Observed Molecular Weight
49 kDa
Calculated molecular weight
50.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03980 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of MEK5 expression in Hela cell lysate.
Specific Publications For Anti-MEK5 Rabbit Monoclonal Antibody (M03980)
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Customer Q&As
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7 Customer Q&As for Anti-MEK5 Rabbit Monoclonal Antibody
Question
Is this M03980 anti-MEK5 Rabbit Monoclonal antibody reactive to the isotypes of MAP2K5?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The immunogen of M03980 anti-MEK5 Rabbit Monoclonal antibody is A synthesized peptide derived from human MEK5 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-20
Question
My question regarding product M03980, anti-MEK5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03980 anti-MEK5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-11
Question
Do you have a BSA free version of anti-MEK5 Rabbit Monoclonal antibody M03980 available?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MEK5 Rabbit Monoclonal antibody M03980 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-30
Question
I was wanting to use your anti-MEK5 Rabbit Monoclonal antibody for WB for human cerebellum on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cerebellum identification?
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
As indicated on the product datasheet, M03980 anti-MEK5 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cerebellum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-21
Question
I am interested in to test anti-MEK5 Rabbit Monoclonal antibody M03980 on human cerebellum for research purposes, then I may be interested in using anti-MEK5 Rabbit Monoclonal antibody M03980 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
The products we sell, including anti-MEK5 Rabbit Monoclonal antibody M03980, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-MEK5 Rabbit Monoclonal antibody M03980. Let me know if you need anything else.
G. Carter
Verified customer
Asked: 2016-07-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-06
Question
I see that the anti-MEK5 Rabbit Monoclonal antibody M03980 works with WB, what is the protocol used to produce the result images on the product page?
R. Bhatt
Verified customer
Asked: 2015-08-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-08-04
