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Product Info Summary
| SKU: | A06836 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-VPS53 Antibody
SKU/Catalog Number
A06836
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-VPS53 Antibody (Catalog # A06836). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
VPS53 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-VPS53 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06836)
Host
Rabbit
Contents
VPS53 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
VPS53 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human VPS53. The immunogen is located within the last 50 amino acids of VPS53.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PACS1 antibody is human, mouse and rat reactive. Multiple isoforms of PACS1 are known to exist. PACS1 antibody is predicted to not cross-react with PACS2.
Reactive Species
A06836 is reactive to VPS53 in Human, Mouse, Rat
Applications
A06836 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
79.653kDa
Background of VPS53
The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of VPS53 in 293 cells with VPS53 antibody at 5 μg/mL.
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Immunofluorescence of VPS53 in 293 cells with VPS53 antibody at 2.5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For VPS53 (Source: Uniprot.org, NCBI)
Gene Name
VPS53
Full Name
Vacuolar protein sorting-associated protein 53 homolog
Weight
79.653kDa
Superfamily
VPS53 family
Alternative Names
FLJ10979; FLJ41112; FLJ61757; HCCS1; hVps53L; MGC39512; pp13624; vacuolar protein sorting 53 (yeast); vacuolar protein sorting 53 homolog (S. cerevisiae); vacuolar protein sorting-associated protein 53 homolog VPS53 HCCS1, PCH2E, hVps53L, pp13624 VPS53 subunit of GARP complex vacuolar protein sorting-associated protein 53 homolog|VPS53, GARP complex subunit|hepatocellular carcinoma suppressor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VPS53, check out the VPS53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VPS53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VPS53 Antibody (A06836)
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No publications found for A06836
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