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Product Info Summary
| SKU: | A11502 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-VPS72 Antibody
SKU/Catalog Number
A11502
Size
100μl
Form
Liquid
Description
Boster Bio Anti-VPS72 Antibody catalog # A11502. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VPS72 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11502)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human VPS72. AA range:131-180
Reactive Species
A11502 is reactive to VPS72 in Human, Mouse
Calculated molecular weight
40.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11502 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blot analysis of lysates from RAW264.7 and NIH/3T3 cells, using VPS72 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using VPS72 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-VPS72 Antibody (A11502)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-VPS72 Antibody
Question
I see that the anti-VPS72 antibody A11502 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-27
Question
Will anti-VPS72 antibody A11502 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
According to the expression profile of cervix carcinoma erythroleukemia, VPS72 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-VPS72 antibody A11502 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-06-12
Question
We are currently using anti-VPS72 antibody A11502 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-15
Answer
The anti-VPS72 antibody (A11502) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-15
Question
Will anti-VPS72 antibody A11502 work on canine WB with embryonic stem cell kidney?
M. Anderson
Verified customer
Asked: 2018-11-20
Answer
Our lab technicians have not validated anti-VPS72 antibody A11502 on canine. You can run a BLAST between canine and the immunogen sequence of anti-VPS72 antibody A11502 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine embryonic stem cell kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-20
Question
Is this A11502 anti-VPS72 antibody reactive to the isotypes of VPS72?
W. Parker
Verified customer
Asked: 2017-11-30
Answer
The immunogen of A11502 anti-VPS72 antibody is Synthesized peptide derived from the Internal region of human VPS72. at AA rangle: 100-180. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-30
Question
Does A11502 anti-VPS72 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Jones
Verified customer
Asked: 2013-05-17
Answer
It shows on the product datasheet, A11502 anti-VPS72 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-05-17
