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Product Info Summary
| SKU: | A06649-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-VTI1a Antibody
SKU/Catalog Number
A06649-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-VTI1a Antibody (Catalog # A06649-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
VTI1a antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-VTI1a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06649-1)
Host
Rabbit
Contents
VTI1a antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
VTI1a antibody was raised against a 19 amino acid peptide near the center of human VTI1a. The immunogen is located within amino acids 50 - 100 of VTI1a.
Cross-reactivity
VTI1a antibody is human, mouse and rat reactive. At least two isoforms of VTI1a are known to exist; this antibody will detect both isoforms. VTI1a antibody is predicted to not cross-react with VTI1b.
Reactive Species
A06649-1 is reactive to VTI1A in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
25.2 kDa
Background of VTI1A
Vesicle transport through interaction with t-SNAREs homolog 1 (VTI1a and VTI1b) are involved in vesicular transport from the late endosomes to the trans-Golgi network (1). They are both localized in the trans-Golgi network, with VTI1a also found in the Golgi apparatus and VTI1b in endosomes (2,3). VTI1a mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane and promotes fusion of the lipid bilayers (4). VTI1a may be concerned with increased secretion of cytokines associated with cellular senescence (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06649-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VTI1a antibody can be used for detection of VTI1a by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of VTI1a in human brain tissue lysate with VTI1a antibody at 1 μg/ml.
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Immunohistochemistry of VTI1a in human brain tissue with VTI1a antibody at 5 μg/mL.
Specific Publications For Anti-VTI1a Antibody (A06649-1)
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