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Product Info Summary
| SKU: | A07432 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-VTI1b Antibody
SKU/Catalog Number
A07432
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-VTI1b Antibody (Catalog # A07432). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
VTI1b antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-VTI1b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07432)
Host
Rabbit
Contents
VTI1b antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
VTI1b antibody was raised against a 17 amino acid peptide near the center of human VTI1b. The immunogen is located within amino acids 150 - 200 of VTI1b.
Cross-reactivity
VTI1b antibody is human, mouse and rat reactive. At least three isoforms of VTI1a are known to exist; this antibody will detect all three isoforms. VTI1b antibody is predicted to not cross-react with VTI1a.
Reactive Species
A07432 is reactive to VTI1B in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
26.7 kDa
Background of VTI1B
Vesicle transport through interaction with t-SNAREs homolog 1 (VTI1a and VTI1b) are involved in vesicular transport from the late endosomes to the trans-Golgi network (1). They are both localized in the trans-Golgi network, with VTI1a also found in the Golgi apparatus and VTI1b in endosomes (2,3). It is thought that VTI1b along with the SNARE protein VAMP8 mediates the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential process for autophagy (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07432 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VTI1b antibody can be used for detection of VTI1b by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of VTI1b in HeLa cell lysate with VTI1b antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of VTI1b in human brain tissue with VTI1b antibody at 5 μg/mL.
Specific Publications For Anti-VTI1b Antibody (A07432)
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