Anti-WDR46 Antibody Picoband™

Product Name

Anti-WDR46 Antibody Picoband™

View all WDR46 Antibodies

SKU/Catalog Number

A12816-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-WDR46 Antibody Picoband™ catalog # A12816-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-WDR46 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12816-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human WDR46 recombinant protein (Position: E166-K495).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A12816-1 is reactive to WDR46 in Human

Applications

A12816-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

68.071kDa

Background of WDR46

WD repeat-containing protein 46 is a protein that in humans is encoded by the WDR46 gene. Enables RNA binding activity. Predicted to be involved in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA). Predicted to be located in nucleoplasm. Predicted to be part of small-subunit processome. Predicted to be active in nucleolus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.

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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-WDR12 antibody (A12816-1).
Overlay histogram showing HL-60 cells stained with A12816-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR12 Antibody (A12816-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For WDR46 (Source: Uniprot.org, NCBI)

Alternative Names

BING4FP221; C6orf11; chromosome 6 open reading frame 11; WD repeat domain 46; WD repeat-containing protein 46; WD repeat-containing protein BING4 WDR46 BING4, C6orf11, FP221, UTP7 WD repeat domain 46 WD repeat-containing protein 46|WD repeat-containing protein BING4

For more info on WDR46, check out the WDR46 Infographic

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In this infographic, you will see the following information for WDR46: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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