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Product Info Summary
| SKU: | A04484 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-WDR79 WRAP53 Monoclonal Antibody
SKU/Catalog Number
A04484
Size
100μl
Form
Liquid
Description
Boster Bio Anti-WDR79 WRAP53 Monoclonal Antibody catalog # A04484. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-WDR79 WRAP53 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04484)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
3F8
Isotype
IgG
Immunogen
Purified recombinant human WDR79 protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04484 is reactive to WRAP53 in Human, Mouse, Rat
Applications
A04484 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
59.309kDa
Background of WDR79
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Pennica D., Swanson T.A., Welsh J.W. J.Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis using WDR79 Monoclonal antibody against HeLa, U2OS cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For WRAP53 (Source: Uniprot.org, NCBI)
Gene Name
WRAP53
Full Name
Telomerase Cajal body protein 1
Weight
59.309kDa
Superfamily
TCAB1 family
Alternative Names
FLJ10385; TCAB1WDR79telomerase Cajal body protein 1; WD repeat containing, antisense to TP53; WD repeat domain 79; WD repeat-containing protein 79; WD40 protein Wrap53; WD40 repeat-containing protein encoding RNA antisense to p53; WD-encoding RNA antisense to p53 WRAP53 DKCB3, TCAB1, WDR79 WD repeat containing antisense to TP53 telomerase Cajal body protein 1|WD repeat-containing protein 79|WD repeat-containing protein WRAP53|WD40 protein Wrap53|WD40 repeat-containing protein antisense to TP53|WD40 repeat-containing protein encoding RNA antisense to p53|WRAP53beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WRAP53, check out the WRAP53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WRAP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WDR79 WRAP53 Monoclonal Antibody (A04484)
Hello CJ!
No publications found for A04484
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-WDR79 WRAP53 Monoclonal Antibody
Question
I was wanting to use your anti-WDR79 Monoclonal antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
It shows on the product datasheet, A04484 anti-WDR79 Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
Is a blocking peptide available for product anti-WDR79 Monoclonal antibody (A04484)?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
We do provide the blocking peptide for product anti-WDR79 Monoclonal antibody (A04484). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-23
Question
We need to test anti-WDR79 Monoclonal antibody A04484 on rat leukemic t-cell for research purposes, then I may be interested in using anti-WDR79 Monoclonal antibody A04484 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
The products we sell, including anti-WDR79 Monoclonal antibody A04484, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-16
Question
Would anti-WDR79 Monoclonal antibody A04484 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
According to the expression profile of leukemic t-cell, WRAP53 is highly expressed in leukemic t-cell. So, it is likely that anti-WDR79 Monoclonal antibody A04484 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-07-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-WDR79 Monoclonal antibody A04484. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-11
Question
I see that the anti-WDR79 Monoclonal antibody A04484 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-14
Question
Is this A04484 anti-WDR79 Monoclonal antibody reactive to the isotypes of WRAP53?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
The immunogen of A04484 anti-WDR79 Monoclonal antibody is Purified recombinant human WDR79 protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-12
Question
See below the WB image, lot number and protocol we used for leukemic t-cell using anti-WDR79 Monoclonal antibody A04484. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-06
Question
Will anti-WDR79 Monoclonal antibody A04484 work on goat WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
Our lab technicians have not tested anti-WDR79 Monoclonal antibody A04484 on goat. You can run a BLAST between goat and the immunogen sequence of anti-WDR79 Monoclonal antibody A04484 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-15
Question
Is there a BSA free version of anti-WDR79 Monoclonal antibody A04484 available?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-WDR79 Monoclonal antibody A04484 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-19
Question
We are currently using anti-WDR79 Monoclonal antibody A04484 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
A. Anderson
Verified customer
Asked: 2017-05-22
Answer
The anti-WDR79 Monoclonal antibody (A04484) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-22
Question
Can you help my question with product A04484, anti-WDR79 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Bhatt
Verified customer
Asked: 2017-03-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04484 anti-WDR79 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-08
Question
Does A04484 anti-WDR79 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Zhang
Verified customer
Asked: 2013-03-06
Answer
You can see on the product datasheet, A04484 anti-WDR79 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-03-06
