Anti-WHIP WRNIP1 Antibody

Product Name

Anti-WHIP WRNIP1 Antibody

View all WHIP Antibodies

SKU/Catalog Number

A06521

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-WHIP WRNIP1 Antibody (Catalog # A06521). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-WHIP WRNIP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06521)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06521 is reactive to WRNIP1 in Human

Applications

A06521 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

72.133kDa

Background of WHIP

Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging.  Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process. A second isoform exists (WHIP2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:40,000
IHC: User optimized
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of WRNIP1 using anti-WRNIP1 antibody (A06521).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRNIP1 antigen affinity purified polyclonal antibody (Catalog # A06521) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for WRNIP1.

Click image to see more details

Figure 2. Western blot analysis of WRNIP1 using anti-WRNIP1 antibody (A06521).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRNIP1 antigen affinity purified polyclonal antibody (Catalog # A06521) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for WRNIP1.

Gene/Protein Information For WRNIP1 (Source: Uniprot.org, NCBI)

Alternative Names

ATPase WRNIP1; bA420G6.2; EC 3.6.1; FLJ22526; putative helicase RUVBL; Werner helicase interacting protein 1; Werner helicase-interacting protein 1; WHIPRP11-420G6.2 WRNIP1 CFAP93, FAP93, WHIP, bA420G6.2 WRN helicase interacting protein 1 ATPase WRNIP1|Werner helicase interacting protein 1|putative helicase RUVBL

For more info on WRNIP1, check out the WRNIP1 Infographic

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In this infographic, you will see the following information for WRNIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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