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Product Info Summary
| SKU: | A03606-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-WNT16 Antibody (C-term)
SKU/Catalog Number
A03606-1
Size
80 µl
Description
Boster Bio Anti-WNT16 Antibody (C-term) (Catalog # A03606-1). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-WNT16 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03606-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This WNT16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-265 amino acids from the C-terminal region of human WNT16.
Reactive Species
A03606-1 is reactive to WNT16 in Human
Calculated molecular weight
40.7 kDa
Background of WNT16
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03606-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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WNT16 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane). This demonstrates the WNT16 antibody detected the WNT16 protein (arrow).
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Western blot analysis of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody (C-term) (Cat. #A03606-1). A03606-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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WNT16 Antibody (C-term) (Cat. #A03606-1)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of WNT16 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Specific Publications For Anti-WNT16 Antibody (C-term) (A03606-1)
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