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- Table of Contents
WNT5B antibody (A05836) performs well in ELISA, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Immunohistochemistry of WNT5 in rat colon tissue with WNT5 antibody at 5 μg/mL.
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Anti-WNT5 WNT5B Antibody
WNT5B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
WNT5 antibody can be used for detection of WNT5 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-WNT5 WNT5B Antibody
View all WNT5B Antibodies
View all WNT5B Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
WNT5 antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
WNT5 antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
WNT5 antibody was raised against a 15 amino acid peptide near the center of human WNT5B. The immunogen is located within amino acids 130 - 180 of WNT5.
Cross-Reactivity
WNT5 antibody is human, mouse and rat reactive. WNT5 antibody will detect both WNT5A and WNT5B. At least two isoforms of WNT5B are known to exist; this antibody will detect both isoforms.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
40.3 kDa
Background of WNT5B
WNT5A and WNT5B are near-identical members of the WNT gene family, which consists of structurally related genes which encode secreted signaling proteins that have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis (1,2). WNT5A and WNT5B coordinate chondrocyte proliferation and differentiation by differentially regulating cyclin D1 and p130 expression, as well as chondrocyte-specific Col2a1 expression (3). Up-regulation of WNT5A and WNT5B has been observed in several types of human cancers, suggesting that they may be useful as diagnostic markers or potent therapeutic targets (4).
Cite This Product
Anti-WNT5 WNT5B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05836)
Publications
Application:
Species:
Chen G,Wang Q,Li Z,Yang Q,Liu Y,Du Z,Zhang G,Song Y.Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head.Bone.2020 Apr;133:115258.doi:10.1016/j.bone.2020.115258.Epu
Species: Human
PMID: 32018039
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Product Promise
We promise Anti-WNT5 WNT5B Antibody performs excellently in relevant applications for ELISA, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A05836
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026