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Product Info Summary
| SKU: | M00234-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-XBP-1 Monoclonal Antibody [3H1G4]
SKU/Catalog Number
M00234-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-XBP-1 Monoclonal Antibody [3H1G4] (Catalog # M00234-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
XBP-1 monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-XBP-1 Monoclonal Antibody [3H1G4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00234-1)
Host
Mouse
Contents
XBP-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 3H1G4
Isotype
IgG1
Immunogen
Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.
Reactive Species
M00234-1 is reactive to XBP1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
28.7 kDa
Background of XBP1
X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00234-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 μg/mL.
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Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 μg/mL.
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Immunofluorescence of XBP1 in HepG2 cells with XBP1 antibody at 4 μg/mL.
Specific Publications For Anti-XBP-1 Monoclonal Antibody [3H1G4] (M00234-1)
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