Click image to see more details
Product Info Summary
| SKU: | A08575 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-XEDAR EDA2R Antibody
SKU/Catalog Number
A08575
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-XEDAR EDA2R Antibody (Catalog # A08575). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human.
Storage & Handling
XEDAR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-XEDAR EDA2R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08575)
Host
Rabbit
Contents
XEDAR Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
XEDAR antibody was raised against recombinant human XEDAR.
Reactive Species
A08575 is reactive to EDA2R in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
32.8 kDa
Background of EDA2R
X-linked ectodysplasin-A2 receptor (XEDAR) is a recently isolated member of the tumor necrosis factor receptor family that is highly expressed during embryonic development and binds to ectodysplatin-A2 (EDA-A2). Two predominantly expressed isoforms, XEDAR-s and XEDAR-L, differ by only a 21-amino region at the juxtamembrane region of the cytoplasmic domain. Neither isoform possesses a death domain and both have been shown to act mainly through TRAF3 and TRAF6 to activate the NF-κB and JNK pathways. Cells transfected with XEDAR and treated with EDA-A2 cause the assembly of a secondary complex containing FADD, caspase-8 and caspase-10, leading to the activation caspase-8 and caspase-3, and finally apoptosis. The EDA-A2-induced apoptosis is dependent on caspase-9 activation, as various pharmacological and genetic inhibitors of caspase-8 blocked apoptosis following EDA-A2 treatment.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08575 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
XEDAR antibody can be used for the detection of XEDAR by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of XEDAR in 293 cell lysate with XEDAR antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Click image to see more details
Immunohistochemistry of XEDAR in human skin tissue with XEDAR antibody at 10 μg/mL.
Specific Publications For Anti-XEDAR EDA2R Antibody (A08575)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-XEDAR EDA2R Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-XEDAR EDA2R Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
