Anti-XPB/ERCC3 Antibody Picoband™

Boster Bio Anti-XPB/ERCC3 Antibody Picoband™ catalog # A03103-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A03103-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-XPB/ERCC3 Antibody Picoband™

See all XPB products

SKU/Catalog Number

A03103-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-XPB/ERCC3 Antibody Picoband™ catalog # A03103-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-XPB/ERCC3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03103-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human XPB recombinant protein (Position: Q665-K715).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A03103-1 is reactive to ERCC3 in Human, Mouse, Rat

Applications

A03103-1 is guaranteed for ELISA, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For ERCC3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P19447

Gene ID

2071

Gene Name

ERCC3

Full Name

General transcription and DNA repair factor IIH helicase subunit XPB

Weight

89.278kDa

Superfamily

helicase family

Alternative Names

Basic transcription factor 2 89 kDa subunit; BTF2 p89; BTF2; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; EC 3.6.1; EC 3.6.4.12; ERCC3; excision repair cross-complementing rodent repair deficiency, complementationgroup 3 (xeroderma pigmentosum group B complementing); GTF2H; RAD25; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p89; TFIIH; Xeroderma pigmentosum group B-complementing protein; xeroderma pigmentosum, complementation group B; XPB; XPBC; XPBTFIIH 89 kDa subunit ERCC3 BTF2, GTF2H, RAD25, TFIIH, TTD2, XPB ERCC excision repair 3, TFIIH core complex helicase subunit general transcription and DNA repair factor IIH helicase subunit XPB|BTF2 p89|DNA excision repair protein ERCC-3|DNA repair helicase|DNA repair protein complementing XP-B cells|TFIIH 89 kDa subunit|TFIIH basal transcription factor complex 89 kDa subunit|TFIIH basal transcription factor complex helicase XPB subunit|TFIIH p89|TFIIH subunit XPB|basic transcription factor 2 89 kDa subunit|excision repair cross-complementation group 3|excision repair cross-complementing rodent repair deficiency, complementation group 3|xeroderma pigmentosum group B-complementing protein|xeroderma pigmentosum, complementation group B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ERCC3, check out the ERCC3 Infographic

ERCC3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ERCC3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-XPB/ERCC3 Antibody Picoband™

Question

Do you have a BSA free version of anti-XPB/ERCC3 antibody A03103-1 available?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-XPB/ERCC3 antibody A03103-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-06

Question

Would A03103-1 anti-XPB/ERCC3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

You can see on the product datasheet, A03103-1 anti-XPB/ERCC3 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-15

Question

Is this A03103-1 anti-XPB/ERCC3 antibody reactive to the isotypes of ERCC3?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

The immunogen of A03103-1 anti-XPB/ERCC3 antibody is E. coli-derived human XPB recombinant protein (Position: Q665-K715). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-10

Question

We are currently using anti-XPB/ERCC3 antibody A03103-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

The anti-XPB/ERCC3 antibody (A03103-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-30

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-XPB/ERCC3 antibody A03103-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-24

Question

Please see the WB image, lot number and protocol we used for placenta using anti-XPB/ERCC3 antibody A03103-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-03-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-03-01

Question

I am interested in to test anti-XPB/ERCC3 antibody A03103-1 on mouse placenta for research purposes, then I may be interested in using anti-XPB/ERCC3 antibody A03103-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

N. Moore

Verified customer

Asked: 2017-10-12

Answer

The products we sell, including anti-XPB/ERCC3 antibody A03103-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-10-12

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A03103-1
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