Click image to see more details
Product Info Summary
| SKU: | A03103-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-XPB/ERCC3 Antibody Picoband®
SKU/Catalog Number
A03103-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-XPB/ERCC3 Antibody Picoband® catalog # A03103-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XPB/ERCC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03103-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human XPB recombinant protein (Position: Q665-K715).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03103-1 is reactive to ERCC3 in Human, Mouse, Rat
Observed Molecular Weight
89 kDa
Calculated molecular weight
89.3 kDa
Background of ERCC3
XPB (xeroderma pigmentosum type B) is an ATP-dependent DNA helicase in humans that is a part of the TFIIH transcription factor complex. This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03103-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat testis tissue, mouse testis tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of XPB using anti-XPB antibody (A03103-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPB antigen affinity purified polyclonal antibody (Catalog # A03103-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPB at approximately 89KD. The expected band size for XPB is at 89KD.
Specific Publications For Anti-XPB/ERCC3 Antibody Picoband® (A03103-1)
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Customer Q&As
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7 Customer Q&As for Anti-XPB/ERCC3 Antibody Picoband®
Question
Do you have a BSA free version of anti-XPB/ERCC3 antibody A03103-1 available?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-XPB/ERCC3 antibody A03103-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-06
Question
Would A03103-1 anti-XPB/ERCC3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
You can see on the product datasheet, A03103-1 anti-XPB/ERCC3 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-15
Question
Is this A03103-1 anti-XPB/ERCC3 antibody reactive to the isotypes of ERCC3?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The immunogen of A03103-1 anti-XPB/ERCC3 antibody is E. coli-derived human XPB recombinant protein (Position: Q665-K715). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-10
Question
We are currently using anti-XPB/ERCC3 antibody A03103-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The anti-XPB/ERCC3 antibody (A03103-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-30
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-XPB/ERCC3 antibody A03103-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-24
Question
Please see the WB image, lot number and protocol we used for placenta using anti-XPB/ERCC3 antibody A03103-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-01
Question
I am interested in to test anti-XPB/ERCC3 antibody A03103-1 on mouse placenta for research purposes, then I may be interested in using anti-XPB/ERCC3 antibody A03103-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Moore
Verified customer
Asked: 2017-10-12
Answer
The products we sell, including anti-XPB/ERCC3 antibody A03103-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-12
