Anti-YB1/YBX1 Antibody Picoband™

YB1 antibody

Boster Bio Anti-YB1/YBX1 Antibody Picoband™ catalog # PB9465. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9465
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-YB1/YBX1 Antibody Picoband™

View all YB1 Antibodies

SKU/Catalog Number

PB9465

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-YB1/YBX1 Antibody Picoband™ catalog # PB9465. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-YB1/YBX1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9465)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human YB1 (293-320aa ENPKPQDGKETKAADPPAENSSAPEAEQ), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9465 is reactive to YBX1 in Human, Mouse, Rat

Applications

PB9465 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Background of YB1

YBX1 (Y box binding protein 1), commonly referred to as "YB-1" by researchers, is a human protein. YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414 and contains 18% basic residues and putative nuclear localization signals. The YBX1 gene is mapped on 1p34.2. Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For YBX1 (Source: Uniprot.org, NCBI)

Uniprot ID

P67809

Gene ID

4904

Gene Name

YBX1

Full Name

Y-box-binding protein 1

Weight

35.924kDa

Superfamily

YBX1 family

Alternative Names

BP-8; CBF-A; class II, Y box-binding protein I; CSDA2; CSDB; DBPB CCAAT-binding transcription factor I subunit A; EFI-A; Enhancer factor I subunit A; MDR-NF1; MGC104858; MGC110976; MGC117250; NSEP1 Y-box-binding protein 1; nuclease-sensitive element-binding protein 1; Y box binding protein 1; YB1 DNA-binding protein B; YB-1 Y-box transcription factor; YBX1 YBX1 BP-8, CBF-A, CSDA2, CSDB, DBPB, EFI-A, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1 Y-box binding protein 1 Y-box-binding protein 1|CCAAT-binding transcription factor I subunit A|DNA-binding protein B|Y-box transcription factor|enhancer factor I subunit A|major histocompatibility complex, class II, Y box-binding protein I|nuclease-sensitive element-binding protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on YBX1, check out the YBX1 Infographic

YBX1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for YBX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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6 Customer Q&As for Anti-YB1/YBX1 Antibody Picoband™

Question

Does PB9465 anti-YB1/YBX1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

As indicated on the product datasheet, PB9465 anti-YB1/YBX1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-25

Question

I was wanting to use your anti-YB1/YBX1 antibody for IHC for mouse eye on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse eye identification?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

As indicated on the product datasheet, PB9465 anti-YB1/YBX1 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse eye in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-20

Question

We are currently using anti-YB1/YBX1 antibody PB9465 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

J. Williams

Verified customer

Asked: 2019-02-20

Answer

The anti-YB1/YBX1 antibody (PB9465) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-02-20

Question

Please see the WB image, lot number and protocol we used for eye using anti-YB1/YBX1 antibody PB9465. Please let me know if you require anything else.

S. Miller

Verified customer

Asked: 2017-06-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-02

Question

We need to test anti-YB1/YBX1 antibody PB9465 on mouse eye for research purposes, then I may be interested in using anti-YB1/YBX1 antibody PB9465 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

O. Jackson

Verified customer

Asked: 2017-05-30

Answer

The products we sell, including anti-YB1/YBX1 antibody PB9465, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-05-30

Question

I see that the anti-YB1/YBX1 antibody PB9465 works with IHC, what is the protocol used to produce the result images on the product page?

B. Williams

Verified customer

Asked: 2015-07-30

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-07-30

Size

Conjugation

Total: $315

SKU:PB9465

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PB9465
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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