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- Table of Contents
Plagl1 antibody (A03220-2) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of ANA-1 cells using anti-ZAC/Plagl1 antibody (A03220-2).
Overlay histogram showing ANA-1 cells stained with A03220-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZAC/Plagl1 Antibody (A03220-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing ANA-1 cells stained with A03220-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZAC/Plagl1 Antibody (A03220-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-ZAC/Plagl1 Antibody Picoband®
Plagl1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
Plagl1 antibody was positively detected by WB in:
mouse heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Plagl1 antibody was positively detected by ICC/IF in:
RM1 cell
Plagl1 antibody was positively detected by FC in:
ANA-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse
Immunocytochemistry/Immunofluorescence
5 μg/ml
Mouse
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Mouse
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-ZAC/Plagl1 Antibody Picoband®
View all PLAGL1 Antibodies
View all PLAGL1 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse ZAC/Plagl1 recombinant protein (Position: M1-Q527).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
51 kDa
Background of Plagl1
Zinc finger protein PLAGL1 is a protein that in humans is encoded by the PLAGL1 gene. Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within regulation of gene expression and skeletal muscle cell differentiation. Predicted to be located in Golgi apparatus and nuclear body. Is expressed in several structures, including alimentary system; brain; embryo mesenchyme; heart; and sensory organ. Orthologous to human PLAGL1 (PLAG1 like zinc finger 1).
Cite This Product
Anti-ZAC/Plagl1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03220-2)
Protocols
Western Blot protocol for Plagl1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Plagl1 antibody
Flow Cytometry (FC) protocol for Plagl1 antibody
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Product Promise
We promise Anti-ZAC/Plagl1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03220-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026