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Product Info Summary
| SKU: | A13201-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-ZADH2 Antibody
SKU/Catalog Number
A13201-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ZADH2 Antibody catalog # A13201-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZADH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13201-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ZADH2. AA range:191-240
Reactive Species
A13201-1 is reactive to ZADH2 in Human, Mouse, Rat
Calculated molecular weight
40.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13201-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Western blot analysis of lysates from HUVEC, HT-29, and COLO cells, using ZADH2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ZADH2 Antibody (A13201-1)
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Customer Q&As
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3 Customer Q&As for Anti-ZADH2 Antibody
Question
I was wanting to use your anti-ZADH2 antibody for WB for mouse quadriceps femoris on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse quadriceps femoris identification?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
You can see on the product datasheet, A13201-1 anti-ZADH2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse quadriceps femoris in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-20
Question
Is this A13201-1 anti-ZADH2 antibody reactive to the isotypes of ZADH2?
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
The immunogen of A13201-1 anti-ZADH2 antibody is Synthesized peptide derived from the Internal region of human ZADH2. at AA rangle: 160-240. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-05
Question
We are currently using anti-ZADH2 antibody A13201-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
The anti-ZADH2 antibody (A13201-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-20
