Product Info Summary
SKU: | M00754-1 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | IHC |
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Product info
Product Name
Anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody
SKU/Catalog Number
M00754-1
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (Catalog # M00754-1). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00754-1)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: ZAP70/528 + 2F3.2
Isotype
IgG2a, kappa + IgG2a, kappa
Immunogen
Recombinant full-length human ZAP70 protein (ZAP70/528); Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70 (2F3.2)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00754-1 is reactive to ZAP70 in Human
Applications
M00754-1 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
69.872kDa
Background of ZAP70
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells.Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-ZAP70 Mouse Monoclonal Antibody (ZAP70/528 + 2F3.2).
Protein Target Info & Infographic
Gene/Protein Information For ZAP70 (Source: Uniprot.org, NCBI)
Gene Name
ZAP70
Full Name
Tyrosine-protein kinase ZAP-70
Weight
69.872kDa
Superfamily
protein kinase superfamily
Alternative Names
70 kDa zeta-associated protein; EC 2.7.10; EC 2.7.10.2; mrtle; mur; SRK; SRKFLJ17679; STD; STDFLJ17670; Syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP70; ZAP-70; zeta-chain (TCR) associated protein kinase (70 kD); zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD ZAP70 ADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70 zeta chain of T cell receptor associated protein kinase 70 tyrosine-protein kinase ZAP-70|70 kDa zeta-associated protein|70 kDa zeta-chain associated protein|syk-related tyrosine kinase|zeta chain of T cell receptor associated protein kinase 70kDa|zeta-chain (TCR) associated protein kinase 70kDa|zeta-chain associated protein kinase, 70kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZAP70, check out the ZAP70 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZAP70: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (M00754-1)
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No publications found for M00754-1
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody
Question
My question regarding product M00754-1, anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00754-1 anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-07
Question
Do you have a BSA free version of anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody M00754-1 available?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody M00754-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-09
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody M00754-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-02
Question
Is this M00754-1 anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody reactive to the isotypes of ZAP70?
L. Kulkarni
Verified customer
Asked: 2018-06-14
Answer
The immunogen of M00754-1 anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody is Recombinant full-length human ZAP70 protein (ZAP70/528); Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70 (2F3.2). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-14
Question
I see that the anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody M00754-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-23
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-23
Question
Does anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody M00754-1 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
According to the expression profile of leukemic t-cell, ZAP70 is highly expressed in leukemic t-cell. So, it is likely that anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody M00754-1 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2017-06-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal antibody M00754-1. Let me know if you need anything else.
M. Krishna
Verified customer
Asked: 2014-09-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-09-26